Application of CRISPR (clustered regularly interspaced short palindromic repeats)/Cpf1 system with compounded crRNA in gene editing

Abstract

The invention discloses application of a CRISPR (clustered regularly interspaced short palindromic repeats)/Cpf1 system in gene editing. The CRISPR/Cpf1 system comprises a1), chemosynthetic crRNA or a2), a crRNA expressing carrier. The CRISPR/Cpf1 system can be c1), an LbCRISPR/Cpf1 system, c2), an Lb2CRISPR/Cpf1 system, c3), an FnCRISPR/Cpf1 system or c4), an AsCRISPR/Cpf1 system. Experiments prove that the chemosynthetic crRNA can play an effective role in guidance of Cpf1 cut-editing specific target sites, and efficiency is high. Applying the crRNA expressed by the carrier or the chemosynthetic crRNA to the CRISPR/Cpf1 system has high application value in gene editing.