A NANOPARTICULATE PERSPECTIVE STUDY -BIOLOGICAL EFFECT OF NANOPARTICLE PREPARATION METHOD IN HOMOEOPATHIC MEDICINE

Abstract

Nanotechnology is currently employed as tool to explore the darkest avenues of medical science in several ways. The size of nanomaterials are similar to that of most biological molecules and structures; therefore, nanomaterials can be useful for biomedical research and applications. Sarsaparilla is a tropical plant from genus smilax(smilacaceae) found in temperate, tropical and subtropical zones worldwide. The rhizome,roots,stems and occasionally leaves of Sarsaparilla are used as food and traditional medicine.This plant is known to have anti-inflammatory, antioxidant, antibacterial, antifungal properties. In this study our aim was to investigate antimicrobial and antioxidant activity of the Sarsaparilla as nanomedicine along with its phytochemical analysis. Sarsaparilla mother tincture and potencies prepared based on homoeopathic law of potentisation. Sarsaparilla has capability of precipitating silver nanoparticle from silver nitrate at ambient temperature. The nanoparticles were characterised by UV spectrometer were the peaks obtained at 434nm for mother tincture,251nm for 30C potency, 250nm for 200C potency and 248nm for 1M potency.SEM showed spherical shape upto 200nm size of particle and by FTIR the functional groups were investigated. The phytochemical analysis was showed positive for Flavonoids, Alkaloids, steroids, Saponin, Amino acids, Tannins, cardiac glycosides,Carbohydrate, Anthraquinone glycosides.The synthesised silver nanoparticle with sarsaparilla were found to be effective as antimicrobial agents against some important human pathogens like E-coli, Staphylococcus aureus, Bacillus, Klebseilla which cause disease and also found as a good antioxidant agent.