BIOINFORMATICS APPLICATIONS NOTE doi:10.1093/bioinformatics/btm100 Structural bioinformatics
A web server for discriminating the stability of proteins and predicting their stability changes upon single amino acid substitutions from amino acid sequence, developed using decision tree coupled with adaptive boosting algorithm, and classification and regression tree, respectively.
Abstract
UNLABELLED We have developed a web server, iPTREE-STAB for discriminating the stability of proteins (stabilizing or destabilizing) and predicting their stability changes (delta deltaG) upon single amino acid substitutions from amino acid sequence. The discrimination and prediction are mainly based on decision tree coupled with adaptive boosting algorithm, and classification and regression tree, respectively, using three neighboring residues of the mutant site along N- and C-terminals. Our method showed an accuracy of 82% for discriminating the stabilizing and destabilizing mutants, and a correlation of 0.70 for predicting protein stability changes upon mutations. AVAILABILITY http://bioinformatics.myweb.hinet.net/iptree.htm. SUPPLEMENTARY INFORMATION Dataset and other details are given.