CREATININE AND CREATINE DETERMINATIONS THE OCCURRENCE OF CREATINE
Int,erference with the usual calorimetric procedures for determining crcatinine and creatine in the urine of diabetics and of those undergoing Aarvation has long been attributed to the presence of acetone bodies and glucose, but the conversion of glucose and other sugars to interfering acids during the hydrolysis of creatine introduces an error which makes the creatininecreatinc value qucstionablc.
Abstract
Int,erference with the usual calorimetric procedures for determining crcatinine and creatine in the urine of diabetics and of those undergoing Aarvation has long been attributed t.o the presence of acetone bodies and glucose. The conversion of glucose and other sugars to interfering acids during the hydrolysis of creatine introduces an error which makes the creatininecreatinc value qucstionablc. The presence of acetone bodies in the various possible proportionsrendcrs the quantitiesof creatinine and creatine both unccrt,ain, since the latter is determined as an atitlition to the former. Whether the effect is an apparent increase in creatinine and a corresponding decrease in creatine value, or the reverse, is a question with evidence on each side. Klercker? and Graham and Poulton3 agree that acetone causes a. decrease in the creatinine results, while Van Hoogenhuyxe and T’crplocgh” and Krause” report a t.emporary elevation, but later disappearance of t.he excess color, allowing the value to become correct. Krause, Wolf and Osterberg,G Rose,’ and Graham and Poulton* found t,hat aceto-acetic acid, or it,s ester, increases the creatinine