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Polymorphism among the antagonistic biocontrol against Aspergillus niger was studied using molecular techniques like RAPD-PCR and 16S rRNA analysis and reflected the genetic diversity among the antagonists by formation of two clusters.
Abstract
Radhaiah et al. Molecular characterization of biocontrol agents using RAPD and 16s rRNA analysis against Aspergillus Niger causing collar rot of groundnut, ABSTRACT Polymorphism among the antagonistic biocontrol against Aspergillus niger was studied using molecular techniques like RAPD-PCR and 16S rRNA analysis. The RAPD banding pattern reflected the genetic diversity among the antagonists by formation of two clusters. A total of 154 reproducible and scorable polymorphic bands ranging approximately as low as 600 bp to as high as 3500 bp were generated with five RAPD primers. The potential antagonists SAMB-17 and SAMB-2 amplified with OPA-20 primer yielded unique bands of 2500bp can be used to develop Sequence Characterized Amplified Region (SCAR) markers. 16S region of rRNA amplification with genus specific 63F and 1387R universal primers produced approximately 1300bp in the fungicide tolerant potential bacterial antagonist. To Cite This Article: Radhaiah A, Nagalakshmi Devamma M. Molecular characterization of biocontrol agents using RAPD and 16s rRNA analysis against Aspergillus Niger causing collar rot of groundnut.