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The invention discloses a water quality detecting device, a water quality monitoring system and an operation method of the water quality monitoring system. The water quality detecting device comprises a data acquiring module for acquiring water quality information of waters, a wireless communication module for obtaining the water quality information acquired by the data acquiring module and sending the water quality information to a water quality monitoring center and a power management module for managing a power supply of the water quality detecting device. The water quality monitoring system comprises at least one water quality detecting device, a water quality monitoring center and a mobile terminal. The water quality detecting device and the water quality monitoring system overcome the defect that original manual water quality detection is influenced by weather and can monitor water quality conditions without influence of the weather in real time. The number of collection points is not limited.