Microbiological Process Report Analytical Microbiology
In these methods, graded concentrations of the substance to be assayed are added to a series of test tubes or flasks containing liquid nutrient medium, and the potency of the sample may be determined by comparing the response of the test organism to the substance being assayed.
Abstract
In these methods, graded concentrations of the substance to be assayed are added to a series of test tubes or flasks containing liquid nutrient medium. This medium is inoculated with the test organism and incubated for a suitable length of time. The response of the test organism, as evidenced by the development of turbidity, is measured by either visual observation of an end point (all-or-none response) or by use of a photoelectric photometer (graded response). By comparing the response of the test organism to the substance being assayed, with that of the test organism to a known standard preparation, the potency of the sample may be determined.