Immunology
It was found out that MHC peptide complex from thymus and kidney blocked the apoptosis in thymocytes, spleenocytes and blood lymphocytes which was induced by dexamethasone, calcium ionophor A23 187, i~-O-tetradecanoyl-i3-acetat and EDTA.
Abstract
We have worked out a method for the extraction of peptides bound to MHC molecules from the tissues of different parenchyma organs. These peptide complexes were investigated by chromatography and its common structure was determined. The purpose of the study was the influence of MHC peptide complexes from thymus and kidney on apoptosis process in thymocytes, spleenocytes and blood lymphocytes by physiologic conditions, the changing of the basic regulative systems and autoimmune response. The apoptosis of lymphocytes was determined by morphology of cell nuclei, chromatin fragmentation, activity of Ca,Mg-endonuclease, characteristic staining by Hoechst 33342, expression of bcl-2, p53 into cells and CDgs/Fas on cell surface. It was found out that MHC peptide complex from thymus and kidney blocked the apoptosis in thymocytes, spleenocytes and blood lymphocytes which was induced by dexamethasone, calcium ionophor A23 187, i~-O-tetradecanoyl-i3-acetat and EDTA. The main difference of these complexes action was an ability of kidney complex to block an apoptosis in intact thymocytes. During experimental autoimmune nephritis treatment by peptide complexes led to the apoptosis of some lymphocyte subpopulations in thymus, spleen and blood. Simultaneously we observed the improvement of kidney morphology and nitrogen excretion. Obtained results display an ability of the using of MHC peptide complexes as potential immunotropic preparations.