An overview of the most recent advances on FTase is presented, with a focus on the application of the hydrolase family 32 glycoside to FOS production.
Fructooligosaccharides (FOS) are considered prebiotic compounds and are found in different vegetables and fruits but at low concentrations. FOS are produced by enzymat‐ ic transformation of sucrose using fructosyltransferase (FTase). Development of new production methods and search for FTase with high activity and stability for FOS production Is an actual research topic. In this article is discussed the most recent advances on FTase and its applications. Different microorganisms have been tested under various fermentation systems in order to identify and characterize new genes codifying for FTase. Some of these genes have been isolated from bacteria, fungi, and plants, with a wide range of percentages of identity but retaining the eight highly conserved motifs of the hydrolase family 32 glycoside. Therefore, this article presents an overview of the most recent advances on FTase and its applications.