Nanoparticles
This work conjugated lectins to superparamagnetic Fe3O4 nanoparticles and utilized them for isolation of dying cells from mixed populations and it was shown that influence of alternating magnetic field resulted in the death of cells with bound biofunctionalized nanoparticles.
Abstract
Maximum 500 characters Previously we found that cells undergoing programmed cell death are characterized by increased expression of particular plasma membrane glycoproteins. Specific lectins (carbohydrate-binding proteins) were used as markers of these cells [1-3]. Here we conjugated lectins to superparamagnetic Fe3O4 nanoparticles and utilized them for isolation of dying cells from mixed populations. Also, it was shown that influence of alternating magnetic field resulted in the death of cells with bound biofunctionalized nanoparticles. Methods used Maximum 300 characters Bioconjugation, light and fluorescent microscopy, cell culturing, affinity chromatography. Related references (max 3) Indicate the impact factor of the cited reference 1. R.Bilyy, R. Stoika. Search for novel cell surface markers of apoptotic cells / Autoimmunity, 2007, V. 40(4) P. 249 253. (invited review, IF ~1.5) 2. R.O. Bilyy, V.O. Antonyuk, R.S. Stoika. Cytochemical study of role of α-D-mannoseand β-D-galactose-containing glycoproteins in apoptosis / Journal of Molecular Histology, 2004. V. 35(8), P. 829-838 (IF ~ 1, formerly Histochemial journal) 3.R.O. Bilyy, R.S. Stoika. Lectinocytochemical detection of apoptotic murine leukemia L1210 cells. / Cytometry, 2003, V 56A(2), P.89-95 (IF ~ 2.7) Related Inventions Disclosures and Patents International PCT Application No. PCT/US2006/42582 Planning grant application (please mark your selection with X) FP7 x NIH Only participating in projects (please mark your selection with X) FP7 X NIH