Top Research Papers on CRISPR

The gene editing technology especially the CRISPR refers to the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) is still being researched but the recent findings show the positive hopes for the scientists.

It is concluded that the proposed solution will not improve the quality of the current system.

crRNAs in Palindromic Repeats (CRISPR)-derived Clustered Regularly Interspaced Short of Required for the Biogenesis and Stability A Complex of Cas Proteins 5, 6, and 7 Is RNA.

The molecular typing approach based on CRISPR polymorphism is not suitable for B. anthracis, but it is possible for us to distinguish B. Anthracis from B. cereus and B. thuringiensis.

Results indicate that there is a correlationship between serogroup obtained using ELISA and the profile generated by CRISPR typing, which has the potential to be applied for the genotyping of Salmonella.

The CRISPR-Cas9 is one of the most efficient tools for genetic engineering in the authors' modern world because of its incredible potential to perform genetic material modification in a wide range of patients with greater efficiency, versatility, and accuracy than before, all the while being more cost-effective.

The authors suggest that the spacer elements are the traces of past invasions by extrachromosomal elements, and hypothesize that they provide the cell immunity against phage infection, and more generally foreign DNA expression, by coding an anti-sense RNA.

This research presents a novel and scalable approach that allows for real-time decision-making in the selection and management of Companion animals for Companion Animal Feeding Protection.

The results show that the CRISPR spacer structure is influenced by and provides a record of the viral challenges that bacteria face, and a population dynamics model is proposed that explains the biological observation that the leader-proximal end of CRISpr is more diversified and theLeader-distal end ofCRISPR is more conserved.

The authors suggest that the spacer elements are the traces of past invasions by extrachromosomal elements, and hypothesize that they provide the cell immunity against phage infection, and more generally foreign DNA expression, by coding an anti-sense RNA.

The current model(s) of the molecular events involved in both the acquisition of immunity and interference stages are presented and recent progress in the knowledge of the regulation of CRISPR/Cas systems is addressed.

This study reviews the recently reported application platforms for screening, diagnosis, and treatment of different diseases based on CRISPR/Cas systems and would be a valuable reference for future genome-editing practices.

The structure, diversity, mechanism of interference and self versus non-self discrimination of CRISPR systems are reviewed and the potential applications of this novel interference system are discussed.

Genetic blood disorders such as sickle cell anemia and β-thalassemia is worldwide problem due transfusion dependency which ultimately led to transfusion related chronic complication such as Hepatitis B & C, HIV and iron overload lead to hemochromatosis etc.

CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats) mulai mendapat perhatian saat Jennifer Doudna dan Emmanuelle Charpentier mendapat hadiah Nobel bidang Kimia tahun 2020 lalu. Meskipun relatif merupakan terobosan baru, terapi CRISPR memiliki potensi besar di bidang medis. Tinjauan ini merupakan pengantar untuk mengenal dan memahami CRISPR sebagai sebuah upaya terapi alternatif di masa depan. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) starts to gain attention when Jennifer Doudna and Emmanuelle Charpentier were awarded Nobel Prize in Chemistry in 2020. CRI...

The main purpose of new medical technologies is to provide diagnosis and treatment of diseases, but the same technologies enable us to become stronger, to live healthier and longer, which can lead to rapid and frequent use of developing technologies beyond purpose and may cause many ethical problems.

It is proposed that the crRNA seed sequence plays a role in the initial scanning of invader DNA for a match, before base pairing of the full-length spacer occurs, which may enhance the protospacer locating efficiency of the E. coli Cascade complex.

This Perspective presents a detailed summary of which micro/nanotechnologies play roles in the advancement of CRISPR diagnosis and how they are involved, and discusses upcoming opportunities and improvements and how micro-nanoteschnologies will continue to play key roles.

This work focuses on the approaches developed since 2013 to decrease the frequency of unwanted mutations (the off-targets) during CRISPR-based gene editing.

Microbiology Insights Volume 16: 1 © The Author(s) 2023 Article reuse guidelines: sagepub.com/journals-permissions DOI: 10.1177/1 7863612 1 78623

It is concluded thatCRISPR gene editing has shown promise in developing precise and genotype-specific therapeutic strategies for DCM, but there are challenges and limitations, such as delivering CRISPR-Cas9 to human cardiomyocytes and the potential for unintended gene targeting.

CRISPR–Cas12a has a higher sensitivity for the detection of KRAS mutations compared with PCR and sequencing analysis, and thus has diagnostic and therapeutic implications, Nevertheless, the technique needs to be validated for its clinical significance in a large and prospective study.

The structure, development, and function in genome editing regulated through the CRISPR/Cas9 is analyzed, and the genes associated with PD pathogenesis and the appropriate modifications to favor PD are explained.

The world's first babies with CRISPR-Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats)-edited genes were born on November 25, 2018 and an assessment of how well they were achieved is discussed.

Data indicated that CRISPR/Cas9 targeting Il2rg causes InDel mutations effectively and generates genetically X-SCID mice, which did not necessarily grant phenotypical alteration, which requires an intensive analysis after establishing a strain to confirm their phenotypes.

The CRISPR presents an interesting genetic marker for comparative and evolutionary analysis of closely related bacterial strains, and the acquisition of new units was shown to happen almost exclusively on one side of the locus.

A package of genes including the CRISPR system i.e. RNA-guided Cas9 endonuclease and guide RNAs along with the malaria resistance gene will be inserted into the germline cells that produce sperm/egg.

A significant correlation was noticed between the CRISPR S1 related to the time of isolation but not between S1, S2, S3 or S4 on theTime of isolation.

Findings suggest that type II-A CRISPR-Cas systems are valuable for genotyping of L. buchneri, a species relevant for commercial silage, bioethanol, and vegetable fermentations.

The results support the proposal that the leader sequence may function as recognition site by having palindromic structures in flanking regions, and the stem-loop secondary structure formed by repeat sequences may function in mediating the interaction between foreign genetic elements and CAS-encoded proteins.

Bioinformatics methods used to analyze the CRISPR structures of Bacillus cereus group genomes demonstrated that stem-loop secondary structure might function in mediating the interaction between foreign genetic elements and CAS-encoded proteins, and predicted two types of RNA secondary structures derived from the repeat sequences.

The presented results on the biogenesis and functions of crRNAs expand the understanding of CRISPR-Cas cellular defenses in P. gingivalis and of horizontal gene transfer in bacteria.

An application of the CRISPR/Cas system is the immunization of industry-relevant prokaryotes (or eukaryotes) against mobile-genetic invasion, and the high variability of theCRISPR spacer content can be exploited for phylogenetic and evolutionary studies.

This article summarizes the discovery process of CRISPR/Cas system and applications of several major CRISpr/Cas derived technologies, aiming to provide a reference for researchers in the field of life science.

The history of biosensors, followed by the current status of nucleic acid-based detection methods, are reviewed, and the current challenges pertaining to CRISPR-based nucleic acids detection are discussed, followedby the recent breakthroughs addressing these challenges.

Cancer remains a global health challenge with high prevalence and mortality rates, imposing significant financial and emotional burdens on affected families. However, hope lies in genetic manipulation, with a focus on innovative approaches to combat genetically linked cancers. Clustered regularly interspaced short palindromic repeats (CRISPR), an adaptive immune system found in various bacteria and archaea, hold immense potential. We searched articles on PubMed Central, Medline, and PubMed databases indexed journals. The keywords from the research topic, i.e., "CRISPR AND genetic therapy," wer...

The aim of the article is to understand what is CRISPR, how it works, different systems ofCRISPR and it's application in medical and dental field.

The CRISPR field would benefit from verifications of self-consistency of data, providing sufficient data for reproduction of experiments, and, in the case of reports of novel assay LoDs, concurrent reporting of the associated kinetic rate constants.

Overall, this study shows that the CRISPR-Cas system positively regulates Salmonella pathogenesis by regulating the expression of different virulence factors.

Using a simple model of the coupled dynamics of phage and bacterial populations, how acquisition rates affect the probability of the bacterial colony going extinct is explored and it is found that the optimal strategy depends on the initial population sizes of both viruses and bacteria.

CRISPR is a cutting-edge technology that has been used extensively in the pre-clinical setting to identify new genetic targets, enhance drug sensitivity, and inhibit cancer progression in animal models.

High-sensitivity and high-specificity biodetection is critical for advancing applications in life sciences, biosafety, food safety, and environmental monitoring. CRISPR/Cas systems have emerged as transformative tools in biosensing due to their unparalleled specificity, programmability, and unique enzymatic activities. They exhibit two key cleavage behaviors: precise ON-target cleavage guided by specific protospacers, which ensures accurate target recognition, and bystander cleavage activity triggered upon target binding, which enables robust signal amplification. These properties make CRISPR/...

The results suggest that the MLST from C. jejuni isolates can be discriminated based on the CRISPR unique spacer sequences and the numbers of spacers, and a database that integrates both MLST sequences andCRISPR sequences and is searchable is greatly in demand for tracking outbreaks and evolution of this bacterium.

The review comprehends multiple facets of the CRISPR-Cas system in Enterobacteriaceae including its diverse attributes, association with genetic features, regulation and gene regulatory mechanisms.

Experiments prove that the chemosynthetic crRNA can play an effective role in guidance of Cpf1 cut-editing specific target sites, and efficiency is high.

A more detailed understanding of the molecular biology of CRISPRs within Taylorella organisms may help elucidate the pathogenic virulence and transmission mechanisms associated with this important equine pathogen.

Some of the many challenges that must be addressed in moving clustered regularly interspaced short palindromic repeats coupled with the nuclease Cas9 therapies to the ophthalmic clinic are discussed here.

CRISPR can provide important information for genotyping and evolutionary research of bacteria, which will help to trace the source of outbreaks and make possible the development of very low cost and high-resolution assays for the systematic typing of any new isolate.

This review focuses on the mechanism of CRISPR/Cas9 technology and its application in stem cell genomic editing, so as to pave the way for promoting rapid application and development of CRisPR/ Cas9 technology.

This review examines the status of applications of CRISPR/Cas in marine research and assesses the prospect of quickly expanding the deployment of this powerful tool to address the myriad fundamental marine biology and biological oceanography questions.