Top Research Papers on CRISPR

The authors suggest that the spacer elements are the traces of past invasions by extrachromosomal elements, and hypothesize that they provide the cell immunity against phage infection, and more generally foreign DNA expression, by coding an anti-sense RNA.

The authors suggest that the spacer elements are the traces of past invasions by extrachromosomal elements, and hypothesize that they provide the cell immunity against phage infection, and more generally foreign DNA expression, by coding an anti-sense RNA.

An application of the CRISPR/Cas system is the immunization of industry-relevant prokaryotes (or eukaryotes) against mobile-genetic invasion, and the high variability of theCRISPR spacer content can be exploited for phylogenetic and evolutionary studies.

Differences in the habitats of enterococcal species may be related with the results observe in distribution of CRISPRs systems, especially in E. faecalis strains isolated from animal feces, compared to food samples.

It is demonstrated that CRISPR/Cas9 can act as a highly specific, efficient, heritable tool for genome manipulation in B. dorsalis and this has significance for gene function research and genetic control of pests.

Findings provided direct evidence that MMP9 is important in the viability, proliferation, and metastasis of cutaneous SCC cells, and serves as a positive foundation for future CRISPR-based targeted anti-cancer therapies in treating skin cancer and other forms of malignancies that involve MMPs as the key determinants.

Eventually, in acute pneumonia and peritonitis mouse models, the sgRNA-I/L@ZS nanosystem could combat bacteria and protect tissues from infection and had marked suppressive effects on inflammation and promoted healing and proliferation of infected tissues.

This Perspective presents a detailed summary of which micro/nanotechnologies play roles in the advancement of CRISPR diagnosis and how they are involved, and discusses upcoming opportunities and improvements and how micro-nanoteschnologies will continue to play key roles.

The results indicate that Salmonella strains present in smaller dairy herds (<500 head) are specific to that farm and new Salmonellosis strains may emerge over time.

The molecular typing approach based on CRISPR polymorphism is not suitable for B. anthracis, but it is possible for us to distinguish B. Anthracis from B. cereus and B. thuringiensis.

CRISPR-based genotyping proves to be a promising tool to complement traditional serotyping methods, enhancing the molecular epidemiology of salmonellosis and potentially leading to better management and control strategies for this pathogen.

The results suggest that the original CRISPR/Cas9 system from the bacterial immune system is not necessarily the best option for the induction of HDR in genome editing and indicate that the modulation of the kinetics of conformational checkpoints of Cas9 can optimize the HDR/NHEJ ratio.

The preliminary data suggest that the combined cell transfection of gene target-specific CRISPR/Cas9 and RNAi to knockdown the NHEJ pathway is a viable and efficient approach to produce precise genetically modified goat donor cells carrying mono- and biallelic knock-ins of large size transgene constructs for use in cloning by SCNT.

It is hypothesised that the CRISPR/Cas9 system can be used to efficiently produce genetically modified embryo models in the pig that will answer key questions about the complex molecular dialogue between conceptus and maternal uterus.

Microbiology Insights Volume 16: 1 © The Author(s) 2023 Article reuse guidelines: sagepub.com/journals-permissions DOI: 10.1177/1 7863612 1 78623

CRISPR has great potential as a future therapeutic modality for coronary artery disease, where this technology is able to control various CAD risk factors and can trigger the formation of new blood vessels to overcome coronary artery blockade, but this very potential technology still requires ethical concern studies in order to be able to maintain human sustainability.

This article summarizes the discovery process of CRISPR/Cas system and applications of several major CRISpr/Cas derived technologies, aiming to provide a reference for researchers in the field of life science.

A more detailed understanding of the molecular biology of CRISPRs within Taylorella organisms may help elucidate the pathogenic virulence and transmission mechanisms associated with this important equine pathogen.

ATCC 29803 possesses genomic components required to express the type II-A CRISPR/Cas system, which potentially functions as an RNA-guided endonuclease.

It is concluded thatCRISPR gene editing has shown promise in developing precise and genotype-specific therapeutic strategies for DCM, but there are challenges and limitations, such as delivering CRISPR-Cas9 to human cardiomyocytes and the potential for unintended gene targeting.