Autoradiographic identification of cerebral and cerebellar cortical neurons accumulating labeled gamma-aminobutyric acid (3H-GABA)
Experimental Brain ResearchPublished 1 March 1972
T. H�kfelt, �. Ljungdahl
Citations246
SJR quartileQ3
SJR score0.64
SNIP0.80
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
Abstract
Small amounts of labeled gamma-aminobutyric acid (3H-GABA), a putative neurotransmitter, were injected stereotaxically into the cerebral and cerebellar cortices of rats pretreated with aminooxyacetic acid (AOAA), a drug which prevents GABA breakdown. After fixation by aldehyde perfusion the tissue was processed for light and electron microscopic autoradiography. Specific autoradiographic uptake patterns were observed in both cortices. A high activity was found mainly over stellate and basket cells in the cerebellar cortex and over stellate cells in the cerebral cortex. Also probable Golgi cells seem to accumulate 3H-GABA. Pyramidal cells and Purkinje cells, on the other hand, showed a low activity. Thus, uptake and retention of 3H-GABA in the brain areas studied seem to be related to interneurons supposed to have an inhibitory function. This is consistent with the view that GABA serves as the major inhibitory transmitter substance in mammalian cortical areas.
Keywords
NeuroscienceBiochemistry, Genetics and Molecular Biology
The Journal of Cell BiologyTHE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY
25,027 Citations1963Edward S. Reynolds
The stain reported here differs from previous alkaline lead stains in that the chelating agent, citrate, is in sufficient excess to sequester all lead present, and is less likely to contaminate sections.
The Journal of Cell BiologyIMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODS
10,250 Citations1961John H. Luft
Epoxy embedding methods of Glauert and Kushida have been modified so as to yield rapid, reproducible, and convenientembedding methods for electron microscopy.
The Cerebellum as a Neuronal Machine
3,234 Citations1967John C. Eccles, Masao Ito +1 more
The Journal of Cell BiologyHIGH-RESOLUTION AUTORADIOGRAPHY
1,074 Citations1962Lucien G. Caro, Robert P. van Tubergen
Methods used in obtaining high resolution in autoradiography, with special emphasis on the technique of electron microscopic autoradography, are described, together with control experiments designed to establish the optimum conditions or procedures.
Brain ResearchSynaptic patterns on different cell types in the different laminae of the cat visual cortex. An electron microscope study
951 Citations1968Marc Colonnier
A device for attachment to an upright post on a bed of a pick-up truck or other vehicle for gripping one end of a tie-down rope to apply tension to the rope to hold pipe or other load on the vehicle when the rope passes over the load and as the opposite end of the rope is secured to the vehicle.
Experimental Brain ResearchThe action of γ-Aminobutyric acid on cortical neurones
618 Citations1967K. Krnjević, Susan M. Schwartz
It is concluded that GABA could be the main cortical inhibitory transmitter in cats under pentobarbitone anaesthesia because the reversal potentials for the action of GABA and the IPSP are approximately similar.
Journal of NeurochemistryTHE UPTAKE OF [<sup>3</sup>H]GABA BY SLICES OF RAT CEREBRAL CORTEX
544 Citations1968Leslie L. Iversen, M J Neal
The GABA uptake system may represent a possible mechanism for the inactivation of GABA or some related substance at inhibitory synapses in the cortex.
Cell and Tissue ResearchIn vitro studies on central and peripheral monoamine neurons at the ultrastructural level
501 Citations1968Tomas H�kfelt
Evidence was obtained that so-called small granular vesicles are characteristic of central and peripheral monoamine neurons, and that these vesicle represent the main storage sites of the monoamines.
Proceedings of the National Academy of SciencesGlial Cell Function: Uptake of Transmitter Substances
463 Citations1971Fritz A. Henn, Anders Hamberger
Results are interpreted as evidence that the glial cell is involved in limiting the extracellular build-up of substances that might trigger synaptic transmission by removing any transmitters that may diffuse out of the synaptic cleft during the transmission of impulses.
ScienceLocalizing Tritiated Norepinephrine in Sympathetic Axons by Electron Microscopic Autoradiography
442 Citations1962David Wolfe, Lincoln T. Potter +2 more
Following intravenous infusion of tritiated norepinephrine, rat pineals were prepared for combined autoradiography and electron microscopy and Concentrations of photographic grains were observed only over regions of preterminal autonomic axons containing granulated vesicles, thereby directly demonstrating uptake of norpinephrine into these axons and strongly suggesting that their granulatedVesicles contain nore Pinephrine.
Experimental Brain ResearchPharmacological properties of the postsynaptic inhibition by Purkinje cell axons and the action of ?-aminobutyric acid on Deiters neurones
354 Citations1967Kenta Obata, M. Ito +2 more
The effects of various drugs applied intravenously or into the fourth ventricle and their effects upon the inhibition exerted by Purkinje cell axons were appraised by both extra- and intracellular recording from Deiters neurones, suggesting the possibility that GABA acts as a natural transmitter.
NatureLocalizing 3H-GABA in Nerve Terminals of Rat Cerebral Cortex by Electron Microscopic Autoradiography
305 Citations1971Floyd E. Bloom, Leslie L. Iversen
Brain ResearchGlutamate decarboxylase in inhibitory neurons
293 Citations1970Frode Fonnum, Jon Storm‐Mathisen +1 more
There is a high concentration of GAD, and consequently of GABA, within the Purkinje axon terminals, and this finding, together with the observations that GABA mimics the action of their natural transmitter and that GABA is released on stimulation of the cerebellar cortex, points to GABA as the transmitter ofPurkinje cells.
Brain ResearchAutoradiography of some suspected neurotransmitter substances: GABA glycine, glutamic acid, histamine, dopamine, and pl-dopa
276 Citations1971B. Ehinger, B. Falck
The experiments demonstrate that in central nervous tissue such as the retina, certain classes of cells preferentially accumulate glycine or GABA, presumably into two different sets of cells, and suggests that a similar role for glycine is worthy of further consideration.
Amino Acid Transmitters
232 Citations1970D.R. Curtis, Graham A.R. Johnston
This analysis focuses on synapses where a transmitter or “mediator” function of amino acids is considered to be very likely and amino acids well suited to this function.
Progress in brain researchThe Primate Cerebellar Cortex: A Golgi and Electron Microscopic Study
216 Citations1967Clement A. Fox, Dean E. Hillman +2 more
The capricious method of Golgi, which now and then reveals the external morphology of a nerve cell and all its processes more completely than any other technique, has been most successful when applied to the cerebellar cortex.
Experimental Brain ResearchSome properties of unresponsive cells in the cerebral cortex
211 Citations1967K. Krnjević, Susan M. Schwartz
It is suggested that unresponsive cells are neuroglia; that the depolarizing actions of GABA and ACh indicate a change in electrogenic active transport, and that glia may remove transmitters from the extracellular space.
Journal of NeurochemistrySUBCELLULAR DISTRIBUTION OF ENDOGENOUS AND [<sup>3</sup>H] γ‐AMINOBUTYRIC ACID IN RAT CEREBRAL CORTEX
153 Citations1969M J Neal, Leslie L. Iversen
The Journal of Cell BiologyLOCALIZATION OF THE SITES OF γ-AMINOBUTYRIC ACID (GABA) UPTAKE IN LOBSTER NERVE-MUSCLE PREPARATIONS
144 Citations1971Paula M. Orkand, Edward A. Kravitz
Both light and electron micrographs showed dense accumulations of label over Schwann and connective tissue cell cytoplasm; muscle was lightly labeled, but axons and terminals were almost devoid of label.
The Journal of Cell BiologyGLUTAMATE UPTAKE BY A STIMULATED INSECT NERVE MUSCLE PREPARATION
138 Citations1970Isabelle R. Faeder, Miriam M. Salpeter
Gl glutamate uptake by isolated cockroach nerve muscle preparations was investigated by means of chemical and electron microscope radioautographic techniques and it was found that the preparation had a high affinity for glutamate and that nerve stimulation enhanced glutamate uptake.
The Structural Design of the Neocortex
130 Citations1965Marc Colonnier
The cerebral cortex is a sheet of gray matter usually described as composed of layers of cells tangential to the pial surface, but the individual layers and their subdivisions are not always clear.
Brain ResearchLight and electron microscopic autoradiography on spinal cord slices after incubation with labeled glycine
127 Citations1971Tomas Hökfelt, Å. Ljungdahl
ScienceElectron-Microscopic Autoradiography of Rat Hypothalamus after Intraventricular H <sup>3</sup> -Norepinephrine
121 Citations1966G K Aghajanian, Floyd E. Bloom
Tritiated norepinephrine was injected into the lateral ventricles of rats, and its localization in the hypothalamus was determined by light and electron-microscopic autoradiography.
The Journal of Cell BiologyINTRANEURAL DISTRIBUTION OF EXOGENOUS NOREPINEPHRINE IN THE CENTRAL NERVOUS SYSTEM OF THE RAT
121 Citations1970Laurent Descarries, Bernard Droz
It is suggested that exogenous norepinephrine bound to macromolecular complexes is present in all parts of catecholaminergic neurons and mainly concentrated within presynaptic axons and synaptic terminals of various morphological types.
Annual Review of PhysiologyCNS at the Cellular Level: Identity of Transmitter Agents
115 Citations1970Catherine O. Hebb
The purpose of this review is to discuss recent evidence relating to the identification of certain substances as central neuro transmitters and to consider some of the pathways in which they may func tion.
NatureSynaptosomes: Different Populations storing Catecholamines and Gamma-aminobutyric Acid in Homogenates of Rat Brain
94 Citations1968Leslie L. Iversen, Solomon H. Snyder
Knowledge of synaptic organization could be greatly advanced if a differential morphology for nerve terminals using various transmitters could be established.
Cellular and Molecular Life SciencesAutoradiographic identification of rabbit retinal neurons that take up GABA
82 Citations1970B. Ehinger
La technique autoradiographique constate que l'acide γ-aminobutyrique est accumulé principalement dans la couche plexiforme interne et dans certaines cellules de the couche de cellules ganglionnaires et of the couches amacrines.
The Journal of Comparative NeurologyStellate cells of the rat parietal cortex
82 Citations1971Alan Peters
Perikaryal cytoplasm is thick, the granular endoplasmic reticulum is often arranged in parallel arrays, and the type of synapse with a side cleft and a prominent postsynaptic density is most common.
Progress in brain researchUptake of [3H]noradrenaline and γ-[3H]Aminobutyric Acid in Isolated Tissues of Rat: An Autoradiographic and Fluorescence Microscopic Study
67 Citations1971Tomas Hökfelt, Å. Ljungdahl
This chapter presents a comparison of the localization of exogenous and endogenous noradrenaline, by means of autoradiography and fluorescence histochemistry, according to the method of Falck and Hillarp.
The Journal of Cell BiologyAUTORADIOGRAPHY OF SOLUBLE MATERIALS
60 Citations1964O. L. Miller, G. E. Stone +1 more
An autoradiographic method is developed by which the presence of soluble intracellular materials can be determined for individual cells and it should be applicable to the assay of various soluble radioactive materials in a variety of cell types under many experimental conditions.
Journal of MicroscopyA modified aldehyde perfusion technique for preventing certain artifacts in electron microscopy of the central nervous system
47 Citations1970Robert L. Schultz, Norman M. Case
Cell and Tissue ResearchThe effect of anisotonic media upon cellular ultra-structure in fresh and fixed rat brain
24 Citations1965Richard M. Torack, Mary Lou Dufty +1 more
The permeability of plasma membranes in the brain appears to be less altered by HAA than by glutal or osmium tetroxide, and the relationship of these findings to a demonstration of the extracellular space in HAA fixed tissues is discussed.