Chemotaxis in Escherichia coli: associations of protein components
BiochemistryPublished 30 September 1980
Daniel Chelsky, Frederick W. Dahlquist
Citations39
SJR quartileQ1
SJR score1.18
SNIP0.73
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
Interactions between protein components of the chemotaxis mechanism in Escherichia coli were investigated by using the cleavable cross-linking reagent, dithiobis(succinimidyl propionate), to demonstrate that MCP I and MCP II exist as functional tetramers.
Abstract
Interactions between protein components of the chemotaxis mechanism in Escherichia coli were investigated by using the cleavable cross-linking reagent, dithiobis(succinimidyl propionate). Two methods were used to allow detection of chemotaxis-specific proteins in intact cells. The first method was to program their synthesis in the presence of [35S]methionine using lambda E. coli hybrid phages which carry the chemotaxis genes. The second method was to label endogenous methyl-accepting chemotaxis proteins (MCP's), with the methyl donor S-adenosyl-L-[methyl-3H]methionine, after permeabilizing the cells with EGTA. Physical associations between proteins were analyzed, after cross-linking, by two dimensional NaDodSO4-polyacrylamide gel electrophoresis. Both labeling methods demonstrate that MCP I and MCP II exist as functional tetramers. Other proteins involved with chemotaxis were found to form dimers and higher polymers. Phage-directed products of cheW, cheX, motA, and cheA formed dimers. CheB and hag products formed multimers. A number of apparent interactions between different gene products were detected as well. Products of cheB, cheW, cheZ, motA, and motB were found to form complexes with other gene products. Included are results consistent with interactions between the products of cheB and cheZ.
Keywords
ChemistryNeuroscienceBiochemistry, Genetics and Molecular Biology
NatureCleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
251,640 Citations1970Ulrich K. Laemmli
Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
European Journal of BiochemistryA Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels
8,651 Citations1974William M. Bonner, Ronald A. Laskey
A simple method for detecting 3H in polyacrylamide gels by scintillation autography (fluorography) using X-ray film, which is ten times more sensitive than conventional autoradiography of isotopes with higher emission energies.
Analytical BiochemistryMolecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate
1,725 Citations1971Richard T. Swank, Kenneth D. Munkres
Because of the importance of intrinsic charge and conformation, the system, although allowing a first approximation in molecular weight determination, may also be applicable to peptide “mapping,” particularly for “insoluble” peptide mixtures with prominent hydrophobic association, such as encountered in cellular membranes, viruses, and proteolytic digests.
Journal of Molecular BiologyDNA replication and the division cycle in Escherichia coli
970 Citations1967D.J. Clark, O. Maaløe
It is concluded that at a given site of synthesis the rate of replication is constant under these growth conditions, and that the measured doubling in rate of synthesis reflects a doubling in the number of sites.
Annual Review of BiochemistryChemotaxis in Bacteria
517 Citations1975Julius Adler
The present review will restrict itself to the recent work on chemotaxis in Escherichia coli and Salmonella typhimurium.
Journal of Biological ChemistryAn Approach to Nearest Neighbor Analysis of Membrane Proteins
364 Citations1974Kuan Wang, Frederic M. Richards
The observed cross-linking patterns of broken ghosts and intact cells treated with these membrane-permeable reagents are almost identical except for the addition of hemoglobin complexes in the latter.
Journal of BacteriologyComplementation analysis and deletion mapping of Escherichia coli mutants defective in chemotaxis.
327 Citations1978John S. Parkinson
Motile, but generally nonchemotactic mutants of Escherichia coli were examined for complementation and recombination with specialized lambdafla transducing phages, finding mutants defective in cheA, cheW, cheX, or cheY function swam smoothly, whereas cheB or cheZ mutants exhibited very high tumbling rates.
Proceedings of the National Academy of SciencesIdentification of a protein methyltransferase as the cheR gene product in the bacterial sensing system.
306 Citations1977R. W. Springer, Daniel E. Koshland
In mutants of the generally nonchemotactic cheR class of Salmonella typhimurium, the methylase is not required for tumbling but appears to be essential for maintaining the appropriate rate constants and levels of the regulator of the chemotactic response.
Proceedings of the National Academy of SciencesA protein methylesterase involved in bacterial sensing.
245 Citations1978Jeffry B. Stock, Daniel E. Koshland
This finding indicates that reversible methylation acts as a control mechanism and that both a methyltransferase and a protein methylesterase are instrumental in bacterial sensing.
Proceedings of the National Academy of SciencesIdentification of a methyl-accepting chemotaxis protein for the ribose and galactose chemoreceptors of Escherichia coli.
180 Citations1979Hisato Kondoh, C. B. Ball +1 more
The discovery of a methyl-accepting chemotaxis protein that functions in the transfer of receptor signals from these two binding proteins to the flagella in Escherichia coli represents a promising base for further study of the linkage between chemoreceptors and flageella in bacteria.
Proceedings of the National Academy of SciencesChemotaxis in <i>Escherichia coli</i> : Methylation of <i>che</i> gene products
159 Citations1977M. Silverman, Melvin I. Simon
Three chemotaxis-specific genes in Escherichia coli, cheM, cheD, and cheZ, are methylated and it is suggested that they may be involved in both an initial process that reflects the instantaneous state of the chemoreceptors and in an integrative, adaptive process.
Journal of Molecular BiologyIn vitro formation of flagella-like filaments and other structures from flagellin
142 Citations1964Dinah Abram, Henry Koffler
The results indicate that the primary structure of the flagellin molecules determines their configuration assumed under various conditions and the manner in which they reassemble to larger polymers in a specific environment.
Proceedings of the National Academy of SciencesIdentification of a gene for the alpha-subunit of RNA polymerase at the str-spc region of the Escherichia coli chromosome.
94 Citations1975S. Richard Jaskunas, Richard R. Burgess +1 more
A structural gene for the alpha-subunit of RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase; EC 2.7.6) has been identified and mapped between spcA and trkA, near 64 min on the E. coli chromosome.
Annual Review of GeneticsBEHAVIORAL GENETICS IN BACTERIA
94 Citations1977John S. Parkinson
A Model of Stimulus Transduction is presented and Implications of the Model are explained and the implications of the model are considered.
Journal of BacteriologyLocalization of proteins controlling motility and chemotaxis in Escherichia coli
91 Citations1977Harry Ridgway, M. Silverman +1 more
Differences in the intracellular locations of the che and mot gene prodcuts presumably reflect the functional attributes of these components.
Journal of BacteriologyIdentification of polypeptides necessary for chemotaxis in Escherichia coli
91 Citations1977M. Silverman, Melvin I. Simon
Molecular cloning techniques were used to construct Escherichia coli-lambda hybrids that contained many of the genes necessary for flagellar rotation and chemotaxis and redefined the "cheA" and "cheB" regions.
CellSensory adaptation mutants of E. coli
90 Citations1978John S. Parkinson, Paul T. Revello
The ability of E. coli to adapt to constant levels of attractant and repellent chemicals was studied by examining the patterns of flagellar movement in cells subjected to abrupt concentration changes, and possible mechanisms of stimulus transduction and sensory adaptation during chemotaxis are discussed.
Journal of BacteriologyFunctional homology of chemotaxis genes in Escherichia coli and Salmonella typhimurium
86 Citations1979Anthony DeFranco, John S. Parkinson +1 more
Generally nonchemotactic mutants of Escherichia coli and Salmonella typhimurium were analyzed by interspecies complementation tests to determine the functional correspondence between the che genes of these two organisms, and poor complementation was observed between noncorresponding genes.
Biochemical and Biophysical Research CommunicationsCross-linking studies on a cytochrome c - cytochrome c oxidase complex
64 Citations1978Margaret M. Briggs, Roderick Capaldi
The major product formed with this 18 A bridging bifunctional reagent was a pair containing II+VI, and other cross-linked products were formed involving different subunits of cytochrome c oxidase.
Proceedings of the National Academy of SciencesInteraction of the cheC and cheZ gene products is required for chemotactic behavior in Escherichia coli.
55 Citations1979John S. Parkinson, S. R. Parker
Observation of swimming patterns and flageLLar rotation in cheC cheZ mutants demonstrated that the interaction of these two gene products influences both the spontaneous frequency of flagellar reversals and the ability of the rotational machinery to respond to chemotactic stimuli.
NatureExpression of flagellar genes carried by bacteriophage lambda
40 Citations1976MICHAEL SILERMAN, Philip Matsumura +3 more
To analyse the hierarchy of control and regulation in this system, a series of hybrid transducing phages that carry different combinations of flagellar genes are prepared and one of these phages is described and it is shown that it can be used to study the expression of flagllar gene activities.