BIOPSY OF HUMAN PREIMPLANTATION EMBRYOS AND SEXING BY DNA AMPLIFICATION
The LancetPublished 1 February 1989
Alan H. Handyside, R. Penketh, Robert Winston, J Pattinson, Joy Delhanty, Edward G. D. Tuddenham
Citations487
SJR quartileQ1
SJR score12.11
SNIP22.72
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TL;DR
A single cell was removed from each of 30 human embryos at the 6-10 cell cleavage stage three days after in-vitro fertilisation, and each male embryo was sexed from the DNA by amplification of a repeated sequence specific for the Y chromosome.
Abstract
A single cell was removed, through a hole made in the zona pellucida, from each of 30 human embryos at the 6-10 cell cleavage stage three days after in-vitro fertilisation. A normal proportion of the embryos (37%) developed to the blastocyst stage by day six in culture and 6 hatched from the zona. Each male embryo was sexed from the DNA by amplification of a repeated sequence specific for the Y chromosome. In 15 embryos with the normal two pronuclei the sex was determined also by in-situ hybridisation with a Y specific probe or fluorescent chromosome staining to detect metaphase Y chromosomes; the results of Y specific amplification were confirmed. This approach may be valuable for couples at risk of transmitting X-linked disease.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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