SOME OBSERVATIONS ON NEGATIVELY STAINED MITOCHONDRIA
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TL;DR
A particulate component of mitochondria is described which is observed in negatively stained preparations of unfixed whole mitochondria or their fragments, and appears to be rather labile and it is destroyed or at least rendered unrecognizable by all conventional fixatives used in electron microscopy, especially OsO(4).
Abstract
A particulate component of mitochondria is described which is observed in negatively stained preparations of unfixed whole mitochondria or their fragments. It consists of a roughly spherical particle, approximately 85 A in diameter, found on the surface of the cristae and probably also on the side of the mitochondrial envelope which faces the matrix. It is attached to the underlying membrane by a narrow stalk 40 to 50 A long. The particle appears to be rather labile and it is destroyed or at least rendered unrecognizable by all conventional fixatives used in electron microscopy, especially OsO(4).