Expression, Purification, and Crystallization of the HIV-1 Reverse Transcriptase (RT)
AIDS Research and Human RetrovirusesPublished 1 November 1990
Torsten Unge, Harri Ahola, Ramagauri Bhikhabhai, Kristina Bäckbro, Seved Lövgren, Éva Mária Fenyõ
Citations38
SJR quartileQ3
SJR score0.55
SNIP0.43
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TL;DR
The HIV-1 pol gene proteins (protease, reverse transcriptase, and endonuclease) were expressed in Escherichia coli N4830-1 by the use of the inducible expression vector pWS60 into which the pol gene was inserted.
Abstract
The HIV-1 pol gene proteins (protease, reverse transcriptase, and endonuclease) were expressed in Escherichia coli N4830-1 by the use of the inducible expression vector pWS60 into which the pol gene was inserted. The p66/p51 heterodimer of reverse transcriptase (RT) was isolated in a highly pure and active form. Crystals of the p66/p51 heterodimer were obtained by the vapor diffusion hanging drop technique. The present crystal quality is still not adequate for high resolution X-ray investigation.
Keywords
Immunology and MicrobiologyMedicineBiochemistry, Genetics and Molecular Biology
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