Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor
SciencePublished 13 March 2003
Aaron F. Straight, Amy Cheung, John Limouze, Irene A. Chen, Nick J. Westwood, James R. Sellers
Citations1,413
SJR quartileQ1
SJR score10.42
SNIP6.62
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TL;DR
It is shown that exit from the cytokinetic phase of the cell cycle depends on ubiquitin-mediated proteolysis and continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components.
Abstract
Completion of cell division during cytokinesis requires temporally and spatially regulated communication from the microtubule cytoskeleton to the actin cytoskeleton and the cell membrane. We identified a specific inhibitor of nonmuscle myosin II, blebbistatin, that inhibited contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. Using blebbistatin and other drugs, we showed that exit from the cytokinetic phase of the cell cycle depends on ubiquitin-mediated proteolysis. Continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components.
Keywords
Biochemistry, Genetics and Molecular Biology
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