Suppression of intestinal neoplasia by DNA hypomethylation
CellPublished 1 April 1995Open access
Peter W. Laird, Laurie Jackson‐Grusby, Amin Fazeli, Stephanie Dickinson, Woon‐Won Jung, En Li
Citations714
SJR quartileQ1
SJR score22.61
SNIP7.62
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TL;DR
Results argue against an oncogenic effect of DNA hypomethylation and are consistent with a role for DNA methyltransferase in the generation of the C to T transitions seen at high frequency in human colorectal tumors.
Abstract
We have used a combination of genetics and pharmacology to assess the effects of reduced DNA methyltransferase activity on ApcMin-induced intestinal neoplasia in mice. A reduction in the DNA methyltransferase activity in Min mice due to heterozygosity of the DNA methyltransferase gene, in conjunction with a weekly dose of the DNA methyltransferase inhibitor 5-aza-deoxycytidine, reduced the average number of intestinal adenomas from 113 in the control mice to only 2 polyps in the treated heterozygotes. Hence, DNA methyltransferase activity contributes substantially to tumor development in this mouse model of intestinal neoplasia. Our results argue against an oncogenic effect of DNA hypomethylation. Moreover, they are consistent with a role for DNA methyltransferase in the generation of the C to T transitions seen at high frequency in human colorectal tumors.
Keywords
Biochemistry, Genetics and Molecular Biology
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