Soluble receptors for cytokines and growth factors: generation and biological function
Biochemical JournalPublished 1 June 1994Open access
Stefan Rose‐John, Peter C. Heinrich
Citations774
SJR quartileQ1
SJR score2.06
SNIP0.98
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TL;DR
Cytokines and growth factors are hormone-like molecules which are secreted by many different cells, mostly after stimulation, and play important regulatory roles in the control of the immune response, haematopoiesis, acute-phase response, inflammatory reactions, cell proliferation and tumour growth.
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ChemistryMedicinePharmacology, Toxicology and Pharmaceutics
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609 Citations1990Raymond G. Goodwin
Analysis of the sequence of the IL-7 receptor revealed significant homology in the extracellular domain to several recently cloned cytokine receptors, demonstrating that the IL -7 receptor is a member of a new receptor superfamily.
Journal of Clinical InvestigationInterleukin 1 receptor antagonist. A new member of the interleukin 1 family.
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592 Citations1991David P. Gearing, Catherine J. Thut +7 more
A cDNA clone encoding the human LIF receptor was isolated by expression screening of a human placental cDNA library and suggests a common signal transduction pathway for the two receptors and may help to explain similar biological effects of the two ligands.
European Journal of ImmunologyThe soluble interleukin‐6 receptor is generated by shedding
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The ligand‐binding subunit (gp80) of the human interleukin‐6 receptor (IL‐6R) was transiently expressed in COS‐7 cells and the metabolically labeled protein was shown to be quantitatively released from the membrane within 20 h.
CellA soluble receptor for interleukin-1β encoded by vaccinia virus: A novel mechanism of virus modulation of the host response to infection
483 Citations1992Antonio Alcamı́, Geoffrey L. Smith
Deletion of B15R from vaccinia virus accelerated the appearance of symptoms of illness and mortality in intranasally infected mice, suggesting that the blockade of IL-1 beta by vacciniairus can diminish the systemic acute phase response to infection and modulate the severity of the disease.
The Journal of Experimental MedicineSoluble cytokine receptors are present in normal human urine.
443 Citations1989Daniela Novick, Hartmut Engelmann +2 more
This finding, together with the already known presence of urinary TNF binding proteins and a soluble IL-2-R both in plasma and in urine, indicates that release of soluble cytokine receptors into body fluids is a general phenomenon that occurs under normal physiological conditions.
The Journal of Experimental MedicineShedding of tumor necrosis factor receptors by activated human neutrophils.
438 Citations1990Françoise Porteu, C F Nathan
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BloodMurine anti-interleukin-6 monoclonal antibody therapy for a patient with plasma cell leukemia
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399 Citations1989W R Baumbach, D L Horner +1 more
The results suggest that the mechanism for production of the rat serum GH-binding protein is by alternative splicing of the gene for the rat GH receptor.
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145 Citations1992Meredith J. Layton, Barry Cross +4 more
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127 Citations1993Pascale Roux‐Lombard, Leonardo Punzi +6 more
The balance between TNF and its specific inhibitors may be critical to the biologic outcome mediated by this cytokine.
FEBS LettersDifferential shedding of the two subunits of the interleukin‐6 receptor
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114 Citations1990Judith G. Giri, Robert Newton +1 more
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112 Citations1990C R Maliszewski, Tetsu Sato +6 more
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Journal of Biological ChemistryRecombinant soluble human interleukin-5 (hIL-5) receptor molecules. Cross-linking and stoichiometry of binding to IL-5.
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European Journal of ImmunologyThe CD27 membrane receptor, a lymphocyte‐specific member of the nerve growth factor receptor family, gives rise to a soluble form by protein processing that does not involve receptor endocytosis
92 Citations1992Wil A. M. Loenen, Evert de Vries +4 more
T cell activation via the antigen receptor strongly enhances CD27 membrane expression, suggesting a role for CD27 during T cell differentiation, and in healthy individuals and patients.
Journal of Biological ChemistryA functional soluble extracellular region of the platelet-derived growth factor (PDGF) beta-receptor antagonizes PDGF-stimulated responses.
91 Citations1991D S Duan, Michael J. Pazin +2 more
The soluble extracellular region protein of the PDGFr binds BB-PDGF with high affinity and functions as a specific antagonist of BB- PDGF actions.
CytokineEnhancement of interleukin 6 cytostatic effect on human breast carcinoma cells by soluble IL-6 receptor from urine and reversion by monoclonal antibody
85 Citations1992Daniela Novick
FEBS LettersSoluble interferon‐α receptor molecules are present in body fluids
79 Citations1992Daniela Novick, Batya Cohen +1 more
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BloodInhibition of acute myelogenous leukemia blast proliferation by interleukin-1 (IL-1) receptor antagonist and soluble IL-1 receptors
78 Citations1992Z Estrov, Razelle Kurzrock +7 more
Proceedings of the National Academy of SciencesA soluble, high-affinity, interleukin-4-binding protein is present in the biological fluids of mice.
76 Citations1990Rafael Fernandez-Botrán, E S Vitetta
It is demonstrated that an IL-4-binding protein (IL-4BP) is constitutively present in the biological fluids of mice (serum, ascites, fluid, and urine) and suggests that IL- 4BP plays an important immunoregulatory role in vivo.
European Journal of BiochemistryRecombinant soluble human interleukin‐6 receptor
67 Citations1993Tanja Stoyan, Uwe Michaelis +5 more
The recombinant soluble human interleukin-6 receptor was expressed in Escherichia coli as a non-glycosylated protein comprising the first 339 amino acids after the signal peptide and was shown to exhibit biological activity, i.e. it stimulated acute-phase protein synthesis in the recently established human hepatoma cell line HepG2-IL-6 which does not express the IL-6-binding subunit of the Il-6R complex on the cell surface.
Journal of Biological ChemistryBiochemical properties of the 75-kDa tumor necrosis factor receptor. Characterization of ligand binding, internalization, and receptor phosphorylation.
66 Citations1992Diane Pennica, Vianna T. Lam +6 more
An expression plasmid encoding the human 75-kDa tumor necrosis factor (TNF) type 2 receptor was constructed and used to generate a stable human cell line (293/TNF-R2) overexpressing TNF- R2, demonstrating that the receptor is phosphorylated.
Journal of Biological ChemistryIdentification, purification, and characterization of truncated forms of the human nerve growth factor receptor
63 Citations1989Andrew Zupan, Patricia A. Osborne +4 more
The potential to isolate milligram quantities of human NGFRts allows for model studies of the physicochemical structure of the intact receptor and the generation of polyclonal antibodies to study the biological functions of the NGF receptor.
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The receptor dimerization reported for the EGF holoreceptor appears not to be a function of its external domain, and the asymmetric unit to consist of one receptor-ligand complex.
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57 Citations1992Tilo Andus, Volker Groß +7 more
Levels of soluble tumor necrosis factor receptor p75 were also higher in ascites than in plasma in patients with malignant and infected ascites, but soluble tumor Necrosis factor receptors p75 levels were usually higher in plasma than in ascite in patientswith uncomplicated hepatic ascites.
Journal of Biological ChemistryPurification and biochemical characterization of a soluble human interferon gamma receptor expressed in Escherichia coli.
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Proceedings of the National Academy of SciencesSoluble tumor necrosis factor receptor: inhibition of human immunodeficiency virus activation.
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Journal of Leukocyte BiologyInterferon-γ and Interleukin-4 down-regulate soluble CD14 release in human monocytes and macrophages
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American Journal of Respiratory Cell and Molecular BiologyCell-surface Receptor Shedding: A Means of Regulating Function
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A number of surface molecules present on cells of various lineages are now known to be shed and thereby released into the extracellular milieu, including many of the growth factor receptors and Fc receptors found on leukocytes are shed.
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44 Citations1991Michael Fountoulakis, E.-J. Schlaeǵer +5 more
The soluble mouse interferon gamma receptor produced in the insect/baculovirus expression system may prove useful to study the function of interfer on gamma receptor as an antagonist of endogenous interferons gamma in the treatment of immunological and inflammatory disorders.
European Journal of BiochemistryAnalysis of soluble human and mouse interferon‐γ receptors expressed in eukaryotic cells
40 Citations1992Reiner Gentz, Ashley Hayes +5 more
The extracellular domains of the human and mouse interferon-gamma receptors were produced in insect Spodoptera frugiperda cells infected with recombinant baculoviruses and in mammalian Chinese-hamster-ovary cells and could be useful for structure/function analyses and in vivo studies.
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40 Citations1993Laurence Ozmen, Giorgio Gribaudo +4 more
The capacity of the injected receptor to bind and neutralize the IFN gamma-mediated antiviral activity was assessed in vivo and it was revealed that mice treated with MoIFN-gamma-R neutralized the protective effect of IFN-Gamma.
Journal of Biological ChemistryEvidence for endocytosis-dependent proteolysis in the generation of soluble truncated nerve growth factor receptors by A875 human melanoma cells
36 Citations1991Andrew Zupan, E. Marshall Johnson
Data support the hypothesis that NGFRt is generated by proteolysis of previously intact receptor, and degradation and truncation were retarded by treatment with: 1) the weak base amines, ammonium chloride or methylamine, or 2) the carboxylic ionophore, monensin, all agents that dissipate endosomal/lysosomal proton gradients via alternate mechanisms.
The Journal of ImmunologyIdentification of a soluble IL-2 receptor <i>beta</i> -chain from human lymphoid cell line cells.
33 Citations1990Mitsuo Honda, K Kitamura +3 more
Results suggest that the released IL-2R beta might serve as an immunoregulatory function in IL- 2 dependent both normal and abnormal immune responses.
Journal of Biological ChemistryIntracellular retention of interleukin-6 abrogates signaling.
32 Citations1993Stefan Rose‐John, Heidi Schooltink +4 more
IL-6 accumulated in the cytoplasm and IL-6 retained in the ER failed to induce liver-specific acute-phase protein synthesis in the host cells, indicating that there is no intracellular role for IL- 6 in signal transduction.
Journal of Biological ChemistryGeneration of the truncated form of the nerve growth factor receptor by rat Schwann cells. Evidence for post-translational processing.
29 Citations1991Philip Barker, Freda D. Miller +2 more
Results suggest that the truncated NGF receptor does not arise as a distinct translation product but rather from a post-translational modification of the intact, surface-bound form of the protein.
BloodShedding of transferrin receptor from rat reticulocytes during maturation in vitro: soluble transferrin receptor is derived from receptor shed in vesicles
27 Citations1991CR Chitambar, AL Loebel +1 more
Journal of Molecular BiologyCrystallization and Preliminary Crystallographic Analysis of a TNF-β-55 kDa TNF Receptor Complex
26 Citations1993A. D’Arcy, David W. Banner +7 more
A complex of tumour necrosis factor-beta with the soluble extracellular domain of the human 55 kDa TNF receptor has been crystallized and diffracts to 2.85 A.
The Journal of ImmunologyRegulation of human monocyte cell-surface and soluble CD23 (Fc epsilon RII) by granulocyte-macrophage colony-stimulating factor and IL-3
24 Citations1992Mark R. Alderson, Teresa W. Tough +2 more
IL-3, IL-4, and GM-CSF were found to markedly enhance the expression of CD23 on the surface of elutriated monocytes and to increase levels of sCD23 in monocyte-culture supernatants and may have important implications for the in vivo therapeutic use of these cytokines.
The Journal of ImmunologyCharacterization of the soluble murine IL-2R and estimation of its affinity for IL-2.
23 Citations1988Dror Baran, Mira Korner +1 more
The ability of sIL-2R to bind IL-2 was assessed by measuring the dissociation and the inhibition constant of the molecule for IL-1 and these values coincide and indicate that the affinity of sil- 2R for IL -2 is at least 10-fold lower than the that of low affinity mIL-1R.
Journal of Biological ChemistryBiochemical study of a recombinant soluble interleukin-2 receptor. Evidence for a homodimeric structure
14 Citations1990Y. Jacques, Brigitte Le Mauff +6 more
Data indicated that the T-S-IL-2R was secreted as an homodimer of two noncovalently associated 40-kDa subunits, suggesting that the p55 chains are also associated as homodimers when imbedded in the membrane.
FEBS LettersEGF stimulates the processing and export of a secreted form of EGF receptor
4 Citations1990J. M. Khire, Manjusri Das
It is demonstrated that exogenously added EGF stimulates post‐translational processing and export of this receptor through a pathway that is insensitive to inhibitors of protein synthesis, and the results suggest that EGF may influence one or more of the rate determining steps that control receptor export from endoplasmic reticulum.
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