Prostaglandin E1 inhibits DNA synthesis in arterial smooth muscle cells stimulated with platelet-derived growth factor
AtherosclerosisPublished 1 October 1984
Jan Nilsson, Anders Olsson
Citations121
SJR quartileQ1
SJR score1.76
SNIP1.38
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TL;DR
The effect of prostaglandins on initiation of DNA synthesis in arterial smooth muscle cells stimulated with platelet-derived growth factor was examined and raised the intracellular concentration of cyclic AMP, indicating that the inhibition may be mediated via changes in the levels ofcyclic nucleotides.
Abstract
The effect of prostaglandins (PG) on initiation of DNA synthesis in arterial smooth muscle cells (SMC) stimulated with platelet-derived growth factor (PDGF) was examined. A concentration of 10 ng/ml PGE1 inhibited DNA synthesis, measured as autoradiographically labeled nuclei, by about 70%. Similar results were obtained with PGE2 and PGD2 but at concentrations 10-20 times higher, whereas PGF2 alpha lacked effect. The inhibitory action of the prostaglandins was restricted to the first 6 h of the lag phase. Treatment with PGE1 also raised the intracellular concentration of cyclic AMP, indicating that the inhibition may be mediated via changes in the levels of cyclic nucleotides.
Keywords
ChemistryBiochemistry, Genetics and Molecular Biology
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