Detection of Giardia lamblia by immunofluorescence
Applied and Environmental MicrobiologyPublished 1 February 1983Open access
John L. Riggs, Kent Dupuis, Keita Nakamura, D P Spath
Citations44
SJR quartileQ1
SJR score1.11
SNIP0.99
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Abstract
High-titer immune sera to cysts of Giardia lamblia, produced in guinea pigs, were labeled with fluorescein isothiocyanate. The resulting conjugates were used to detect G. lamblia in stool specimens by fluorescence microscopy. The sera also reacted with cysts of Chilomastix mesnili, but the two organisms could be differentiated by their size.
Keywords
Immunology and Microbiology
Journal of Clinical PathologySerum antibodies and jejunal histology in giardiasis associated with malabsorption.
122 Citations1976Marian J. Ridley, D.S. Ridley
An immunofluorescent test gave positive results in 32/36 cases of giardiasis with malabsorption, and there was a crude correlation between antibody titre and the severity of the histological lesion in the jejunum.
American Journal of Tropical Medicine and HygieneUltrastructure of the Cyst of Giardia Lamblia
98 Citations1977Harley G. Sheffield, Bjarne Bjorvatn
An isolation technique involving filtration and discontinuous density gradient centrifugation was utilized for obtaining Giardia lamblia cysts from human feces and ribbon-like structures and associated microtubules apparently are reorganized into sucking disks upon division of the cyst organism.
Annals of Internal MedicineAn Immunofluorescence Test to Detect Serum Antibodies to <i>Giardia lamblia</i>
83 Citations1980Govinda S. Visvesvara, PHILLIP D. SMITH +2 more
The results indicate that G. lamblia, an intestinal parasite often regarded as noninvasive, induces a systemic antibody response that is specific and reproducible and may be useful in epidemiologic and immunologic studies of giardiasis.