Human Immunodeficiency Virus (HIV) Antigen-Antibody Combination Assays: Evaluation of HIV Seroconversion Sensitivity and Subtype Detection
Journal of Clinical MicrobiologyPublished 30 October 2002Open access
Thoai Duong Ly
Citations82
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Results indicate that although all combination assays can detect antibodies to HIV-1, group M, subtypes A to G, circulating recombinant form (CRF) A/E, and HIV- 1 group O, their sensitivity varied considerably when tested using diluted HIV-2 antibody-positive samples.
Keywords
Immunology and MicrobiologyMedicine
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AIDSSerologic and phylogenetic characterization of HIV-1 subtypes in Uganda
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Journal of Clinical MicrobiologyContribution of Combined Detection Assays of p24 Antigen and Anti-Human Immunodeficiency Virus (HIV) Antibodies in Diagnosis of Primary HIV Infection by Routine Testing
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35 Citations1988George J. Dawson, J. Heller +8 more
The molecularly cloned the gag and env genes of the human immunodeficiency virus and expressed fragments of these genes in Escherichia coli to develop two competitive immunoassays (CIAs), comparable with western blot in detecting antibodies in patients with AIDS or AIDS-related complex that were repeatably reactive in the HIV screening test.
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The p24 gag antigen is expressed in Escherichia coli in order to produce a diagnostic reagent for the detection of virus exposure and the use of bacterially produced antigens for ELISAs gave results that were comparable to those obtained by using antigen isolated from the virus.
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A very well-documented study on the time course of viral antigenemia and seroconversion after human immunodeficiency virus (HIV) transfer by using a simple ELISA procedure is reported.
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Nine ELISA tests and one particle agglutination test for the detection of HIV antibodies were analysed using serial dilutions of Western blot-confirmed positive serum samples, and remarkable differences in sensitivity were observed.