Hydroxyapatite column chromatography in procedures for isolation of purified DNA
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TL;DR
Two procedures are described for the isolation of purified DNA from mammalian tissues by using hydroxyapatite chromatography, a modification of the MUP method of Britten et al. in which some technical difficulties are overcome and the yield and the purity of the DNA are improved.
Abstract
Two procedures are described for the isolation of purified DNA from mammalian tissues by using hydroxyapatite chromatography. They avoid enzyme treatment and are easily carried out in one day. The first one is a modification of the MUP (8 m urea-0.24 m sodium phosphate buffer) method of Britten et al. (2) in which some technical difficulties (clogging of the column) are overcome and the yield and the purity of the DNA are improved. The second procedure represents a combination of the classical methods of lysis of muclei with hydroxyapatite chromatography of the DNA and may be especially convenient for the isolation of high-molecular-weight DNA.