A 5S rRNA/L5 complex is a precursor to ribosome assembly in mammalian cells.
The Journal of Cell BiologyPublished 1 March 1988Open access
Joan A. Steitz, Carmen van den Berg, Joseph P. Hendrick, H La Branche-Chabot, Andres Metspalu, Jutta Rinke
Citations175
SJR quartileQ1
SJR score3.36
SNIP1.52
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TL;DR
The protein moiety of the 5S RNP has been identified as ribosomal protein L5, which is known to be released from ribosomes in a complex with 5S after various treatments of the 60S subunit, and indirect immunofluorescence indicates that the L5/5S complex is concentrated in the nucleolus.
Abstract
A novel 5S RNA-protein (RNP) complex in human and mouse cells has been analyzed using patient autoantibodies. The RNP is small (approximately 7S) and contains most of the nonribosome-associated 5S RNA molecules in HeLa cells. The 5S RNA in the particle is matured at its 3' end, consistent with the results of in vivo pulse-chase experiments which indicate that this RNP represents a later step in 5S biogenesis than a previously described 5S*/La protein complex. The protein moiety of the 5S RNP has been identified as ribosomal protein L5, which is known to be released from ribosomes in a complex with 5S after various treatments of the 60S subunit. Indirect immunofluorescence indicates that the L5/5S complex is concentrated in the nucleolus. L5 may therefore play a role in delivering 5S rRNA to the nucleolus for assembly into ribosomes.
Keywords
Biochemistry, Genetics and Molecular Biology
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