Hybridization of mitochondrial ribosomal RNA

Renaturation kinetics showed that the sequence length molecular weight of Neurospora mitochondrial DNA is more than 66 × 106, therefore, the genes for 25 s and 19 s RNA are repeated at least four times in the mitochondrial DNA.

Abstract

Mitochondrial and cytoplasmic ribosomal RNA's of Neurospora crassa were hybridized with mitochondrial or nuclear DNA which was immobilized on nitrocellulose filters. The 25 s and 19 s mitochondrial RNA's were complementary to 6.1% and 2.8% of the mitochondrial DNA, respectively. The 28 s and 18 s cytoplasmic RNA's were complementary to 0.67 and 0.33% of the nuclear DNA. Renaturation kinetics showed that the sequence length molecular weight of Neurospora mitochondrial DNA is more than 66 × 106. Therefore, the genes for 25 s and 19 s RNA are repeated at least four times in the mitochondrial DNA.