A Consensus Sequence for the Adenovirus-2 Genome
Published 1 January 1986
Richard J. Roberts, Göran Akusjärvi, Peter Aleström, Richard Gelinas, T Gingeras, Daniela Sciaky
Citations92
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TL;DR
The complete sequence of 35,937 nucleotides for the Adenovirus-2 genome is presented in a fully annotated form and represents a consensus derived by combining sequence data from many different laboratories.
Abstract
The complete sequence of 35,937 nucleotides for the Adenovirus-2 genome is presented in a fully annotated form. This sequence represents a consensus derived by combining sequence data from many different laboratories.
Keywords
MedicineBiochemistry, Genetics and Molecular BiologyEnvironmental Science
CellCoincidence of the promoter and capped 5′ terminus of RNA from the adenovirus 2 major late transcription unit
390 Citations1978Edward B. Ziff, Ronald M. Evans
Strong homologies at cap sites and splice points suggest that for the noted cases, the virus and cell share closely related mechanisms for mRNA 5' end synthesis and splicing.
NatureStructure of two spliced mRNAs from the transforming region of human subgroup C adenoviruses
381 Citations1979Michel Perricaudet, Göran Akusjärvi +2 more
The results enable us to predict the primary sequence of two related polypeptides from region E1A of human subgroup C adenoviruses, and to study the structure of early ad2 mRNAs at the nucleotide level using molecular cloning procedures to amplify the appropriate mRNA sequences.
Journal of Biological ChemistryNucleotide sequences from the adenovirus-2 genome.
327 Citations1982T Gingeras, Daniela Sciaky +8 more
The sequence of 15,441 nucleotides from the adenovirus-2 genome has been determined and includes the regions between coordinates 0-32% and 89-100%, as well as the two virus-associated RNAs and the leader sequences for the major late mRNAs.
Journal of Molecular BiologyPromoters and heterogeneous 5′ termini of the messenger RNAs of adenovirus serotype 2
288 Citations1981Carl C. Baker, Edward B. Ziff
A model for Ad2 cap site recognition is proposed in which RNA polymerase scans short DNA segments for purine starts, and is discussed in the context of EIV mRNAs of Ad2 and Ad5, which differ in their promoter region DNA sequence.
CellThe gene for polypeptide IX of adenovirus type 2 and its unspliced messenger RNA
197 Citations1980Peter Aleström, Göran Akusjärvi +4 more
A 606 base pair segment of adenovirus type 2 DNA which includes the gene for polypeptide IX and its flanking regions has been sequenced and direct analysis of its capped oligonucleotide show that the gene and its mRNA are colinear.
CellNucleotide sequence analysis of the leader segments in a cloned copy of adenovirus 2 fiber mRNA
185 Citations1979Sayeeda B. Zain, Joe Sambrook +4 more
This prototype sequence, which has also been recognized at or near the splice points in other eucaryotic systems, is possibly part of a larger unit which serves as a recognition site for specific excision-ligation events that ultimately lead to the production of mature mRNAs.
Nucleic Acids ResearchNucleotide sequence of the EcoRI E fragment of adenovirus 2 genome
178 Citations1981Jacques Hérissé, Francis Galibert
The entire nucleotide sequence of the Ad.2 EcoRI E fragment has been determined using the Maxam and Gilbert method, allowing deduction of 80% of the aminoacid sequence of this protein.
NatureControls of RNA splicing and termination in the major late adenovirus transcription unit
169 Citations1981Göran Akusjärvi, Håkan Persson
CellMessenger rna for the ad2 dna binding protein: dna sequences encoding the first leader and heterogeneity at the mRNA 5′ end
166 Citations1979Carl C. Baker, Jacques Hérissé +3 more
The structure of the first leader of the DBP mRNA and the flanking sequences in the DNA are described, and the implications of these findings for the mechanism of specifying mRNA 5' ends are discussed.
Proceedings of the National Academy of SciencesStructure of genes for virus-associated RNAI and RNAII of adenovirus type 2.
158 Citations1980Göran Akusjärvi, Michael B. Mathews +3 more
The data suggest that the two RNA genes may have arisen by duplication of an ancestral gene and that the folding of the RNA chain may be of importance for the function of VA RNAs.
Nucleic Acids ResearchStructure and organization of the gene coding for the DNA binding protein of adenovirus type 5
154 Citations1981W. Kruijer, F.M.A. van Schaik +1 more
From the nucleotide sequence of the DBP gene it can be derived that DBP contains 529 amino acid residues and has an actual molecular weight of 59,049 daltons.
Journal of Molecular BiologyThe nucleotide sequences at the termini of adenovirus-2 DNA
126 Citations1979John R. Arrand, Richard J. Roberts
No significant self-complementarity exists within the inverted terminal repetition, making terminal initiation of DNA replication via a self-priming mechanism unlikely, however, the terminal A + T-rich region followed immediately by a very G + C -rich region is consistent with other schemes for adenovirus-2 DNA replication.
Nucleic Acids ResearchNucleotide sequence of adenovirus 2 DNA fragment encoding for the carboxylic region of the fiber protein and the entire E4 region
121 Citations1981Jacques Hérissé, Muriel Rigolet +2 more
The position within the nucleotide sequence of various open reading frames and of several consensus splicing sequences was correlated with the location by EM and Sl digestion of the E4 mRNA, allowing to suggest an additional splicing event in the maturation process of i or f mRNA and to deduce the structure of most E4 RNA.
Nucleic Acids ResearchThe sequence of the 3′ non-coding region of the hexon mRNA discloses a novel adenovirus gene
117 Citations1981Göran Akusjärvi, Jan Zabielski +2 more
The sequence revealed unexpectedly that the 3' non-coding region of the hexon mRNA contains a 609 nucleotide long uninterrupted translational reading frame following a potential initiator AUG.
Proceedings of the National Academy of SciencesMultiple mRNA species for the precursor to an adenovirus-encoded glycoprotein: identification and structure of the signal sequence.
98 Citations1980Håkan Persson, Hans Jörnvall +1 more
Early region 3 of the adenovirus type 2 genome encodes three proteins with molecular weights of 16,000, 14,500, and 14,000 (E2/16, E3/14.5, and E3-3.5) and a partial amino acid sequence was determined after radioactive labeling in vitro and this sequence can be aligned with a known DNA sequence.
GeneNucleotide sequence of the EcoRI-F fragment of adenovirus 2 genome
98 Citations1979Francis Galibert, Jacques Hérissé +1 more
Using the DNA sequence method of Maxam and Gilbert the entire nucleotide sequence of the adenovirus 2 EcoRI-F fragment was determined and information contained in that nucleotide sequences is interpreted with respect to the mapping and processing of the three mRNAs partly encoded by the EcoRI -F fragment.
CellSequence analysis of adenovirus DNA: Complete nucleotide sequence of the spliced 5′ noncoding region of adenovirus 2 hexon messenger RNA
93 Citations1979Göran Akusjärvi, Ulf Pettersson
The complete nucleotide sequence of the 5' noncoding region of the adenovirus 2 hexon messenger RNA has been established by sequence analysis of reverse transcripts by comparing the established mRNA sequence with the genomic sequences for the first and third leader segments.
Nucleic Acids ResearchNucleotide sequence of the EcoR1 D fragment of adenovirus 2 genome
85 Citations1980Jacques Hérissé, G. Courtois +1 more
Journal of Biological ChemistryDNA sequence analysis of the region encoding the terminal protein and the hypothetical N-gene product of adenovirus type 2.
80 Citations1982Peter Aleström, Göran Akusjärvi +2 more
The complete nucleotide sequence of a 5783-base pair segment of adenovirus type 2 DNA, located between map coordinates 15.8 and 31.7, is determined and reveals two translational reading frames encoded by the viral 1-strand which have remarkably large coding capacities.
Journal of Biological ChemistryThe Nucleotide Sequence of a Low Molecular Weight Ribonucleic Acid from Cells Infected with Adenovirus 2
74 Citations1971Keiji Ohe, Sherman M. Weissman
Nucleic Acids ResearchCharacterization of the major mRNAs from adenovirus 2 early region 4 by cDNA cloning and sequencing
68 Citations1984G A Freyer, Y. Katoh +1 more
The sequences at the splice junctions of many early region 4 (E4) mRNAs from adenovirus 2 (Ad2) were determined by analysis of cDNA clones, and nine unique sequence patterns were identified and representative clones were completely sequenced.
Journal of VirologySplice junctions in adenovirus 2 early region 4 mRNAs: multiple splice sites produce 18 to 24 RNAs
66 Citations1984Michael Tigges, Heschel J. Raskas
The splice junctions in adenovirus 2 early region 4 (E4) mRNAs were localized and eight acceptor and two donor splice sites utilized in the deletion of the intron from the 3' portion of these m RNAs were detected.
Journal of Biological ChemistryStudies of low molecular weight RNA from cells infected with adenovirus 2. I. The sequences at the 3' end of VA-RNA I.
64 Citations1977M.L. Celma, J. Pan +1 more
The nucleotide sequence of a DNA fragment overlapping the 3' end of VA-RNA I has been determined and the DNA sequence shows some similarity to known transcription termination sequences in prokaryotic systems.
Journal of VirologyGene and mRNA for precursor polypeptide VI from adenovirus type 2
62 Citations1981Göran Akusjärvi, Håkan Persson
A 1,040-base-pair-long sequences of adenoviruses type 2 DNA which encodes the complete gene for precursor polypeptide VI (pVI) is presented, suggesting the evolution of pVI and hexon as separate proteins by the introduction of a termination codon and selection of a new splice acceptor site in an ancestral fused polypeptic chain.
Proceedings of the National Academy of SciencesNucleotide sequence at the junction between the coding region of the adenovirus 2 hexon messenger RNA and its leader sequence.
59 Citations1978Göran Akusjärvi, U. Pettersson
A 139-base-pair sequence of adenovirus 2 DNA that is located immediately leftwards of the cleavage site for endonuclease Sma I at position 51 is determined.
VirologyAmino-terminal sequence of adenovirus type 2 proteins: Hexon, fiber, component IX, and early protein 1B-15K
57 Citations1980Carl W. Anderson, James B. Lewis
The partial amino-terminal amino acid sequence has been determined for four adenovirus 2 proteins: hexon, fiber, component IX, and early protein E1B-15K, which has identified the initiation sites for protein synthesis.
Journal of Biological ChemistryStudies of low molecular weight RNA from cells infected with adenovirus 2. II. Heterogeneity at the 5' end of VA-RNA I.
55 Citations1977M.L. Celma, J. Pan +1 more
It is concluded that transcription of VA-RNA I can initiate at two different nearby sites, except for the 5'-terminal products.
Journal of Molecular BiologySequence analysis of adenovirus DNA
54 Citations1979Göran Akusjärvi, Ulf Pettersson
The regions on the adenovirus type 2 genome which encode the three segments of the common tripartite leader of late adenvirus messenger RNA have been identified and sequenced and exhibit a striking similarity to the corresponding regions of eukaryotic genes.
Biochemical and Biophysical Research CommunicationsNucleotide sequence at the inverted terminal repetition of adenovirus type 2 DNA
52 Citations1979Mitsuru Shinagawa, R. Padmanabhan
The nucleotide sequence of the inverted repetition present at the termini of adenovirus type 2 DNA is determined and it is exactly identical in sequence at both termini.
Proceedings of the National Academy of SciencesPredicted structure of two adenovirus tumor antigens.
51 Citations1980Michel Perricaudet, J Moullec +1 more
Early adenovirus type 2(Ad2) mRNA sequences have been cloned by using the pBR322 plasmid as a vector and it was possible to deduce the structure of a 13S and a 22S mRNA.
Journal of Molecular BiologyThe molecular structure of the 9 S mRNA from early region 1A of adenovirus serotype 2
51 Citations1983Anders Virtanen, Ulf Pettersson
Proceedings of the National Academy of SciencesGene and protein sequences of adenovirus protein VII, a hybrid basic chromosomal protein.
51 Citations1983Michael T. Sung, Ting Cao +2 more
The displacement and replacement of protein VII during the viral infectious cycle in the host cell appears to mimic the biology of nucleoprotamine during the processes of spermatogenesis and fertilization.
Nucleic Acids ResearchNucleotide sequence of the gene encoding adenovirus type 2 DNA binding protein
49 Citations1982W. Kruijer, F.M.A. van Schaik +1 more
The nucleotide sequence of the gene encoding adenovirus type 2 (Ad2) DNA binding protein (DBP) is determined and the complete amino acid sequence of Ad2 DBP has been deduced.
Nucleic Acids ResearchAccurate and efficient in vitro splicing of purified precursor RNAs specified by early region 2 of the adenovirus 2 genome
49 Citations1983Carlos J. Goldenberg, Scott D. Hauser
Findings provide evidence that a nuclear extract prepared from MOPC-315 mouse myeloma cells is capable of accurate and efficient splicing of E2 RNA precursors.
Journal of VirologyMapping of a 14,000-dalton antigen to early region 4 of the human adenovirus 5 genome
47 Citations1983Jeffrey Downey, David Rowe +3 more
An early 14,000-dalton (14K) antigen of adenovirus 5, hitherto designated 10.5K and thought to be from early region 1 (E1), has been shown to be a product of region E4 on the following evidence.
Journal of VirologyPolyadenylic acid addition sites in the adenovirus type 2 major late transcription unit
46 Citations1983J Moullec, Göran Akusjärvi +6 more
The polyadenylation sites of the mRNAs that belong to the five families at the nucleotide level are defined and the hexanucleotide sequence AAUAAA is present at the 3' end of all late adenovirus type 2 m RNAs.
Cold Spring Harbor Symposia on Quantitative BiologyBiogenesis, Structures, and Sites of Encoding of the 5' Termini of Adenovirus-2 mRNAs
44 Citations1980Carl C. Baker, Edward B. Ziff
Journal of Molecular BiologySequences from the beginning of the fiber messenger RNA of adenovirus-2
44 Citations1979B.Sayeeda Zain, Richard J. Roberts
Small restriction fragments, from around co-ordinate 86.6 on the adenovirus-2 genome, have been used as primers for direct DNA sequence analysis by Sanger's (Sanger et al., 1977) chain termination method with Ad2† DNA as template.
Biochemical and Biophysical Research CommunicationsAn acetylated N-terminus of adenovirus type 2 hexon protein
44 Citations1974Hans Jörnvall, Harriet Ohlsson +1 more
An acetylated N-terminus of adenovirus type 2 hexon protein was characterized using radioactively labeled protein and mass spectrometry, with a radioactive peptide recovered in good yield.
Nucleic Acids ResearchTwo initiation sites for adenovirus 5.5S RNA
43 Citations1978Björn Vennström, Ulf Pettersson +1 more
In isolated nuclei, alpha-amanitin inhibits all 5.5S RNA transcription at a concentration of 200 microgram/ml, indicating that both initiation sites are recognized by RNA polymerase III.
Journal of Molecular BiologyDetermination and analysis of adenovirus-2 DNA sequences which may include signals for late messenger RNA processing
42 Citations1979S Zain, T Gingeras +3 more
Nucleotide sequences have been determined for regions of the adenovirus-2 genome from which the three principal leaders of late messenger RNA are transcribed and revealed specific repeated primary and secondary structural features at both the 5′ and 3′ ends of each of the conserved RNA segments present in adenova-2 late mRNA transcripts.
VirologySequence analysis of adenovirus DNA I. Nucleotide sequence at the carboxy-terminal end of the gene for adenovirus type 2 hexon
40 Citations1978Göran Akusjärvi, Ulf Pettersson
The nucleotide sequence of a 210 base-pair long fragment of adenovirus type 2 DNA has been determined and the hexon gene is terminated by a single UAA stop codon which starts 136 nucleotides to the left of the cleavage site for endonuclease Bam HI.
Nucleic Acids ResearchAn adenovirus agnogene
39 Citations1982Anders Virtanen, Peter Aleström +3 more
S1 nuclease mapping and sequence analysis of cDNA copies of adenovirus mRNA demonstrated that the established sequence includes the i-leader, which is spliced to the 5'-end of certain adanovirus mRNAs, suggesting that the i, unlike other adenvirus leader segments, is used for translation.
GeneStructure of three spliced mRNAs from region E3 of adenovirus type 2
38 Citations1983Per Stålhandske, Håkan Persson +3 more
The results demonstrate, moreover, that certain splice points which are used to generate the major E3 mRNAs are also used to splice the supplementary leader segments to the fibre mRNA at late times after infection.
Journal of Molecular BiologyPoly(A) sites of adenovirus serotype 2 transcription units
36 Citations1982Nigel W. Fraser, Carl C. Baker +2 more
This paper analyzes 3′-terminal sequences of messenger RNAs from five early transcription units, and messenger RNA from the L2, L3 and L4 families of the major late transcription unit, and presents a model for sequence recognition during formation of 3′ ends, and discusses autonomy of poly(A) site function.
VirologyStructure and function of adenovirus DNA binding protein: Comparison of the amino acid sequences of the Ad5 and Ad12 proteins derived from the nucleotide sequence of the corresponding genes
33 Citations1983W. Kruijer, F.M.A. van Schaik +2 more
The analysis of the Ad5 DBP gene and adjacent regions by determination of the sequence of the first leader in late DBP mRNA's and the splice point between the tripartite leader and the main body of the mRNA encoding the 100-kDa protein has been extended, suggesting that both domains of DBP are subjected to different evolutionary pressures.
Journal of VirologyPolypeptide structure and encoding location of the adenovirus serotype 2 late, nonstructural 33K protein
33 Citations1983E A Oosterom-Dragon, Carl W. Anderson
Radiochemical microsequence analysis of selected tryptic peptides of the adenovirus type 2 33K nonstructural protein has revealed the precise region of the genomic nucleotide sequence that encodes this protein, allowing a prediction of the complete amino acid sequence for the 33K protein.
GeneThe nucleotide sequence of mRNA for the Mr 19000 glycoprotein from early gene block III of adenovirus 2
31 Citations1982Chulbul M. Ahmed, Rita Chanda +2 more
The cytoplasmic poly(A)RNA from early stages of infection of HeLa cells by adenovirus 2 (Ad2), was used to synthesize cDNA and the clone pE22 which contained a copy of the mRNA coding for the Mr 19 000 glycoprotein was characterized in detail.
Proceedings of the National Academy of SciencesMapping a eukaryotic promoter: a DNA sequence required for in vivo expression of adenovirus pre-early functions.
31 Citations1981Timothy F. Osborne, Richard E. Schell +3 more
It is shown that a portion of the adenovirus 2 sequence between 23 and 38 base pairs upstream from the mRNA cap site is required for expression of the pre-early region, which includes the Goldberg-Hogness box-like sequence T-A-T-T
Journal of VirologyPeptide maps and N-terminal sequences of polypeptides from early region 1A of human adenovirus 5
31 Citations1984Jeffrey Downey, Carole Evelegh +2 more
Peptide maps and N-terminal sequences were obtained for E1A products from the Ad5 deletion mutant dl1504, which lacks the normal initiator codon, and these polypeptides are initiated at the next ATG, 15 codons downstream in the wild-type sequence.
Journal of Biological ChemistryStudies of low molecular weight RNA from cells infected with adenovirus 2. III. The sequence of the promoter for VA-RNA I.
30 Citations1977J. Pan, M.L. Celma +1 more
VA-RNA I is one of the very few RNA species produced in animal cells whose transcriptional initiation site is known precisely and the nucleotide sequence of the DNA preceding the 5' end of VA- RNA I is analyzed.
GeneThe nucleotide sequence of the gene for protein IVa2 and of the 5' leader segment of the major late mRNAs of adenovirus type 5
29 Citations1981C.P. van Beveren, J. Maat +2 more
The nucleic acid data allow us to predict an Mr of 50873 for the IVa2 protein of Ad5, which is close to the experimentally determined value of 50000 (Persson et al., 1979), and the DNA sequence described here also includes the information for the 5'-terminal leader segment of the major late mRNAs of Ad 5.
The EMBO JournalLeader arrangement in the adenovirus fiber mRNA.
25 Citations1982Mathias Uhlén, C. Svensson +5 more
Four oligonucleotides, complementary to the adenovirus 2 (ad2) fiber mRNA were chemically synthesized and used as primers to study the 5′ end of that messenger, and four differently spliced leaders were characterized.
Journal of VirologySequence arrangement and protein coding capacity of the adenovirus type 2 "i" leader
25 Citations1983Eileen Falvey, Edward B. Ziff
It is suggested that the i leader constitutes an Ad-2 coding sequence whose novel position within the leader of major late transcription unit messengers allows it to be translated in preference to coding sequences in mRNA main bodies.
VirologyProteins encoded near the adenovirus late messenger RNA leader segments
24 Citations1983James B. Lewis, Carl W. Anderson
Cell-free translation of three adenovirus 2 DNA cloned into the single-strand phage M13 produced proteins that encoded within the first and second segments of the tripartite major late leader, within the "i"-leader segment, and immediately preceding the third leader segment.
VirologyRecombination in adenovirus: DNA sequence analysis of crossover sites in intertypic recombinants
20 Citations1983Vivien Mautner, M. E. G. Boursnell
The nucleotide sequence of the adenovirus type 5 genome has been determined for a 620-bp region that spans the C terminus of the pVI gene and the N termini of the hexon gene, and compared to the adensvirus type 2 DNA sequence.
Journal of Biological ChemistryMolecular processing of adenovirus proteins.
18 Citations1983Michael T. Sung, Ting Cao +2 more
The analogous constitution of the precursor fragments in Pro-VI and Pro-VII suggests that a common mechanism is implicated in controlling the reorganization of VI and VII during virion assembly.
GeneThe nucleotide sequence of the right-hand terminal SmaI-K fragment of adenovirus type 2 DNA
17 Citations1980Mitsuru Shinagawa, R. Padmanabhan +3 more
The primary structure of the SmaI-K fragment of adenovirus type 2 (Ad2) DNA has been determined and shows a remarkable homology which may be significant in view of the fact that Ad2 and Ad5 DNAs can interchangeably function in the in vitro replication system of Challberg and Kelly (1979).
Nucleic Acids ResearchNucleotide sequences and mapping of novel heterogenous 5′-termini of adenovirus 2 early region 4 mRNA
14 Citations1981S Hashimoto, Michael Pursley +1 more
Ad2 E4 major mRNA species appear to be unique since mRNA molecules initiate at a pyrimidine, perhaps by RNA polymerase stuttering, or they are products of an unusual type of RNA processing.
BiochemistryNucleotide sequences of 5' termini of adenovirus 2 early transforming region Ela and Elb messenger ribonucleic acids
10 Citations1981Shuichi Hashimoto, William S.M. Wold +2 more