Quantitative determination of the glutathione, cysteine, and N-acetyl cysteine conjugates of acetaminophen by high-pressure liquid chromatography
Analytical BiochemistryPublished 1 November 1977
Alan R. Buckpitt, Douglas E. Rollins, Sidney D. Nelson, Ronald B. Franklin, J.R. Mitchell
Citations87
SJR quartileQ3
SJR score0.50
SNIP0.63
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TL;DR
A quantitative method for the estimation of the glutathione, Cysteine, and N -acetyl cysteine conjugates of acetaminophen obtained from microsomal incubations has been developed using high-pressure liquid chromatography (HPLC).
Abstract
A quantitative method for the estimation of the glutathione, cysteine, and N-acetyl cysteine conjugates of acetaminophen obtained from microsomal incubations has been developed using high-pressure liquid chromatography (HPLC). As little as 5 ng of the thiol conjugates of [ring-14C]acetaminophen can be measured with good precision from microsomal incubations with minimal sample preparation. High-pressure liquid chromatography offers distinct advantages over the standard separation techniques of thin-layer, paper, or gel filtration chromatography for the separation and quantitation of the polar thiol metabolites of acetaminophen.
Keywords
Biochemistry, Genetics and Molecular BiologyPharmacology, Toxicology and Pharmaceutics
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