Behaviour modification by in vitro mutagenesis of a variable region within the period gene of Drosophila
NaturePublished 1 April 1987
Qiang Yu, Hildur V. Colot, Charalambos P. Kyriacou, Jeffrey C. Hall, Michael Rosbash
Citations130
SJR quartileQ1
SJR score18.29
SNIP10.16
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
The phenotypes of transformed fruit flies, in which the only functional period gene lacks the entire perfect threonine-glycine repeat region, show that the effects of the period gene on the circadian and male courtship song rhythms can be dissociated.
Abstract
The period gene of Drosophila melanogaster, implicated in the control of both the circadian and male courtship song rhythms, is found to be polymorphic. Alleles differ in the length of a region of the gene encoding a series of threonine-glycine repeat units. The phenotypes of transformed fruit flies, in which the only functional period gene lacks the entire perfect threonine-glycine repeat region, show that the effects of the period gene on the circadian and male courtship song rhythms can be dissociated.
Keywords
Agricultural and Biological SciencesNeuroscience
NatureHypervariable ‘minisatellite’ regions in human DNA
3,763 Citations1985Alec J. Jeffreys, Victoria Wilson +1 more
A probe based on a tandem-repeat of the core sequence can detect many highly variable loci simultaneously and can provide an individual-specific DNA ‘fingerprint’ of general use in human genetic analysis.
Proceedings of the National Academy of SciencesClock Mutants of <i>Drosophila melanogaster</i>
2,296 Citations1971Ronald J. Konopka, Seymour Benzer
Three mutants have been isolated in which the normal 24-hour rhythm is drastically changed and all these mutations appear to involve the same functional gene on the X chromosome.
NatureCryptic simplicity in DNA is a major source of genetic variation
690 Citations1986Diethard Tautz, Martin Trick +1 more
It is shown here that nearly all possible simple motifs occur 5–10 times more frequently than equivalent random motifs, and a new computer algorithm reveals the widespread occurrence of significantly high levels of a new type of ‘cryptic simplicity’ in both coding and noncoding DNA.
Journal of Molecular BiologyChromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster
634 Citations1983Welcome Bender, Pierre Spierer +2 more
A chromosomal walk is described that covers 315 X 10(3) base-pairs of DNA from the 87DE region of the third chromosome of Drosophila melanogaster, which includes the DNA for the rosy and Ace loci, which code for xanthine dehydrogenase and acetylcholinesterase.
NatureRestoration of circadian behavioural rhythms by gene transfer in Drosophila
499 Citations1984Thaddeus A. Bargiello, F. Rob Jackson +1 more
It is reported that when a 7.1-kb fragment from a per+ fly, including the sequences encoding the 4.5-kb transcript, is introduced into the genome of a per0 (arrhythmic) fly by P element-mediated transformation, circadian rhythmicity of behaviour such as eclosion and locomotor activity is restored.
CellMolecular analysis of the period locus in Drosophila melanogaster and identification of a transcript involved in biological rhythms
425 Citations1984Pranhitha Reddy, William A. Zehring +5 more
Evidence is discussed, from previously reported genetic and phenotypic analysis of per's function, suggesting that this region may be complex and that several gene products from the per region, including this 0.9 kb transcript, may be involved in the different aspects of normal rhythmicity influenced by this clock gene.
CellP-element transformation with period locus DNA restores rhythmicity to mutant, arrhythmic drosophila melanogaster
391 Citations1984William A. Zehring, David A. Wheeler +5 more
Certain subsegments of the per region, transduced into the genome of arrhythmic pero flies, restore rhythmicity in circadian locomotor behavior and the male's courtship song.
Proceedings of the National Academy of SciencesCircadian rhythm mutations in Drosophila melanogaster affect short-term fluctuations in the male's courtship song.
352 Citations1980C. P. Kyriacou, J C Hall
Both circadian rhythms and a very short, noncircadian oscillation appear to be influenced by the same gene.
Proceedings of the National Academy of SciencesMolecular genetics of a biological clock in <i>Drosophila</i>
243 Citations1984Thaddeus A. Bargiello, Michael W. Young
Physical characterization of a series of chromosomal rearrangements altering per locus activity indicates that DNA affecting behavioral rhythms is found in a 7.1-kb HindIII fragment.
Animal BehaviourThe function of courtship song rhythms in Drosophila
238 Citations1982Charalambos P. Kyriacou, Jeffrey C. Hall
These newly-discovered song cycles produce significant mating enhancement in D. melanogaster and D. simulans, with the females showing a preference for songs which carry both the species-specific IPI and the Species- specific IPI rhythm.
NatureProduct of per locus of Drosophila shares homology with proteoglycans
236 Citations1986F. Rob Jackson, Thaddeus A. Bargiello +2 more
A fragment of DNA of ∼7 kilobases (kb) encoding a 4.5-kb poly(A)+ RNA restores rhyth-micity when transduced into Drosophila carrying mutations5,6 or chromosomal deletions5 of the per locus and the sequence of this biologically active segment of DNA is reported.
NatureA family of unusually spliced biologically active transcripts encoded by a Drosophila clock gene
232 Citations1987Yoav Citri, Hildur V. Colot +5 more
Complementary DNA cloning of the transcripts of the Drosophila clock gene period reveals three distinct transcripts that result from unusual splicing pathways, one involving a CG 3′ splice site and one resulting in the use of two different reading frames in one exon.
NatureChanges in abundance or structure of the per gene product can alter periodicity of the Drosophila clock
229 Citations1987Mary K. Baylies, Thaddeus A. Bargiello +2 more
It is suggested that perl and pers mutants produce hypoactive and hyperactive per proteins, respectively, which are inversely correlated with period length, so that flies with lowest levels of the per product have slow-running biological clocks.
Journal of Biological ChemistryDeletion of clustered O-linked carbohydrates does not impair function of low density lipoprotein receptor in transfected fibroblasts.
206 Citations1986Claire Davis, Åke P. Elhammer +5 more
It is concluded that: 1) the serine- and threonine-rich region of the LDL receptor is the site for addition of clustered O-linked carbohydrates; 2) the receptor contains a small number of isolated chains of O- linked carbohydrates in addition to the clustered chains; and 3) the clustered O -linked carbohydrates are not essential for LDL receptor function in cultured hamster fibroblasts.
Proceedings of the National Academy of SciencesMolecular cloning and sequence analysis of a chondroitin sulfate proteoglycan cDNA.
202 Citations1985Mario A. Bourdon, Å Oldberg +2 more
The identification and DNA sequence of a chondroitin sulfate proteoglycan core protein cDNA is reported and the possibility of detecting mRNAs and genomic sequences for other proteoglycans with a serine-glycine repeat by using this cDNA clone is discussed.
NatureAn unusual coding sequence from a Drosophila clock gene is conserved in vertebrates
196 Citations1985Hee‐Sup Shin, Thaddeus A. Bargiello +3 more
The results of a search for sequences homologous to the per locus DNA in the genomic DNA of several species of vertebrates show an unusual, tandemly repeated sequence forming a portion of the 4.5-kb per transcript is homologously to DNA in chicken, mouse and man.
Journal of NeurogeneticsGerm-Line Transformation Involving DNA from the<i>period</i>Locus in<i>Drosophila melanogaster</i>: Overlapping Genomic Fragments that Restore Circadian and Ultradian Rhythmicity to<i>per<sup>0</sup></i>and<i>per<sup>−</sup></i>Mutants
169 Citations1986Melanie Hamblen, William A. Zehring +8 more
P-element-mediated transformations involving DNA fragments from the period (per) clock gene of Drosophila melanogaster have shown that several subsegments of the locus restore rhythmicity to per0 or per- mutants.
Proceedings of the National Academy of SciencesMolecular mapping of point mutations in the period gene that stop or speed up biological clocks in Drosophila melanogaster.
157 Citations1987Qiang Yu, Alain Jacquier +4 more
The pero1 and the pers mutations in Drosophila melanogaster, which seem to eliminate or speed up, respectively, the clocks underlying biological rhythmicity, were mapped to single nucleotides.
CellThe period clock locus of D. melanogaster codes for a proteoglycan
144 Citations1986Pranhitha Reddy, Agnes C. Jacquier +3 more
To determine whether the per transcript codes for a proteoglycan, a region of its coding sequence was expressed (in bacteria) as part of a fusion protein, which was used to immunize rabbits and detected an antigen that is present in wild-type flies and absent in a per- mutant.
Molecular and General Genetics MGGEffects of dosage alterations at the per locus on the period of the circadian clock of Drosophila
114 Citations1982Randall F. Smith, Ronald J. Konopka
The per+ dosage results and the complementation behavior of pers indicate that the hypermorphic phenotype of pers results from increased activity of the pers gene product rather than an overproduction of per+ product.
NatureLearning and memory mutations impair acoustic priming of mating behaviour in Drosophila
87 Citations1984Charalambos P. Kyriacou, Jeffrey C. Hall
Testing whether prior exposure of females to a rhythmic pulse song speeds up subsequent mating performance reports here that it does, and learning and memory mutations, expressed in the females to whom songs are being played, either ‘block’ or attenuate the effectiveness of acoustical priming.
Molecular and General Genetics MGGMosaic analysis of a Drosophila clock mutant
72 Citations1983Ronald J. Konopka, Steven Wells +1 more
Gynandromorphs that showed mosaicism for an X chromosome carrying a short-period (pers) circadian clock mutation and several visible mutations were produced by two different methods, suggesting that the oscillators in the two halves of the brain were of different genotype and could function independently.
The EMBO JournalEmbryonic expression of the period clock gene in the central nervous system of Drosophila melanogaster.
56 Citations1986Anthony James, John Ewer +3 more
It is suggested that the 4.5‐kb mRNA has a developmental role for establishing mechanisms that are necessary for eventual expression of clock functions.
Cold Spring Harbor Symposia on Quantitative BiologyA Biological Clock in Drosophila
48 Citations1985Michael W. Young, F. Rob Jackson +2 more
It is suggested that the periodicity of circadian rhythms in Drosophila is determined by the level of expression of a per locus protein encoded by the 4.5-kb transcript.