The moss,Physcomitrella patens, transformed with apoaequorin cDNA responds to cold shock, mechanical perturbation and pH with transient increases in cytoplasmic calcium
Transgenic ResearchPublished 1 May 1996
Angela J. Russell, Marc R. Knight, David J. Cove, C. D. Knight, Anthony Trewavas, T L Wang
Citations48
SJR quartileQ1
SJR score0.57
SNIP0.61
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
The transformation of the mossPhyscomitrella patens with the cDNA for apoaequorin is reported, obtained in the wild type which reconstitute the calcium-sensitive luminescent protein aequorinin vivo after incubation in coelenterazine, and continue to grow normally.
Abstract
The gene for apoaequorin has been used previously to indicate cytosolic calcium changes in higher plants. Here we report the transformation of the moss Physcomitrella patens with the cDNA for apoaequorin. Stable transformants were obtained in the wild type which reconstitute the calcium-sensitive luminescent protein aequorin in vivo after incubation in coelenterazine, and continue to grow normally. The wild type responds to cold-shock (0-10 degrees C) with increases in cytosolic calcium. Mechanical perturbation, in the form of touch, also induces transient increases in cytosolic calcium. A smaller response to pH, distinct from the touch response and exhibiting different kinetics, can also be detected.
Keywords
Agricultural and Biological SciencesBiochemistry, Genetics and Molecular Biology
NatureTransgenic plant aequorin reports the effects of touch and cold-shock and elicitors on cytoplasmic calcium
1,170 Citations1991Marc R. Knight, Anthony K. Campbell +2 more
It is shown here that aequorin can be reconstituted in transformed plants and that it reports calcium changes induced by touch, cold-shock and fungal elicitors, which could be valuable for determining the role of calcium in intracellular signalling processes in plants.
CellRain-, wind-, and touch-induced expression of calmodulin and calmodulin-related genes in Arabidopsis
732 Citations1990Janet Braam, Ronald W. Davis
The regulation of thisCalmodulin-related gene family in Arabidopsis suggests that calcium ions and calmodulin are involved in transduction of signals from the environment, enabling plants to sense and respond to environmental changes.
PlantaThigmomorphogenesis: The response of plant growth and development to mechanical stimulation
516 Citations1973M. J. Jaffe
In Mimosa pudica, flower bud production was retarded, as was the growth of the tendrils, leaves, and petioles in Bryonia dioica, and some evidence indicates that thigmomorphogenesis may be mediated by ethylene.
Critical Reviews in Plant SciencesCalcium and Signal Transduction in Plants
365 Citations1993B. W. Poovaiah, Anireddy S. N. Reddy +1 more
Since the last review on the Ca2+ messenger system in 1987, there has been tremendous progress in elucidating various aspects of Ca(2+) -signaling pathways in plants, including demonstration of signal-induced changes in cytosolic Ca2+, cal modulin and calmodulin-like proteins, and increased evidence in support of the role of inositol phospholipids in the Ca( 2+) - Signaling system.
Proceedings of the National Academy of SciencesWind-induced plant motion immediately increases cytosolic calcium.
328 Citations1992Marc R. Knight, Steven M. Smith +1 more
The data suggest that mechanoperception in plant cells may possibly be transduced through intracellular calcium, and that calcium could be central in the control and generation of plant form.
Molecular and General Genetics MGGStable transformation of the moss Physcomitrella patens
259 Citations1991Didier G. Schaefer, J.-P. Zrÿd +2 more
The stable transformation of Physcomitrella patens to either G418 or hygromycin B resistance following polyethylene glycol-mediated direct DNA uptake by protoplasts is reported.
Molecular and General Genetics MGGUptake, integration, expression and genetic transmission of a selectable chimaeric gene by plant protoplasts
229 Citations1985Rüdiger Hain, Priska Stabel +4 more
Genetic analysis of one of the kanamycin resistant plants shows that the chimaeric gene is transmitted to the progeny as a single dominant trait in a Mendelian fashion.
PlantaCytoplasmic free calcium in Riccia fluitans L. and Zea mays L.: Interaction of Ca2+ and pH?
174 Citations1988Hubert Felle
It is concluded that regulation of cytoplasmic pH and free Ca2+ are interrelated and double-barrelled microelectrodes are useful tools for investigations of intracellular ion activities in plant cells.
The Journal of Cell BiologyImaging calcium dynamics in living plants using semi-synthetic recombinant aequorins.
159 Citations1993Marc R. Knight, Nick D. Read +2 more
By using a highly sensitive photon-counting camera connected to a light microscope, for the first time changes in [Ca2+]i in response to cold-shock, touch and wounding in different tissues of transgenic Nicotiana plants are imaged.
Journal of Cell ScienceCell wall oligogalacturonides increase cytosolic free calcium in carrot protoplasts
73 Citations1993J. Messiaen, Nick D. Read +2 more
The data support the view that a special conformation of the oligogalacturonides possessing >9 residues is necessary to induce an elevation in [Ca2+]i of carrot protoplasts and suggest that exogenous Ca2+ is the major source for the rise in this cell wall elicitor.
PlantaPhototropism and polarotropism of primary chloronemata of the moss Physcomitrella patens: responses of the wild-type
62 Citations1983Gareth I. Jenkins, David J. Cove
Simultaneous illumination with a high fluence rate of red light and a low fluence rates of far-red light causes a marked increase in the percentage of filaments growing towards the red light source at the expense of those growing at right angles to it, supporting the hypothesis that in red and far- red light, at least, the responses are controlled by the photoequilibrium of a phytochrome pool.
Regulation of Development in the Moss, Physcomitrella patens
53 Citations1992David J. Cove
Mosses have a number of advantages which gives them considerable potential as systems for understanding plant development at the molecular level, and their effects on development are therefore straightforward to study.
Cell CalciumCytokinin increases intracellular Ca2+ in Funaria: Detection with indo-1
35 Citations1991Sung Ho Hahm, Mary Jane Saunders
Changes in intracellular [Ca2+] ([Ca2-]i) after cytokinin-treatment in protonema cells of the moss Funaria hygrometrica have been measured using the pentapotassium salt of Indo-1 to show differential physiological response of these cell types to hormonal stimulation.