Enzymatic microdetermination of serum free fatty acids
Analytical BiochemistryPublished 1 October 1979
Sakayu Shimizu, Keiko Inoue, Yoshiki Tani, Hideaki Yamada
Citations216
SJR quartileQ3
SJR score0.50
SNIP0.63
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TL;DR
A reliable, simple, and rapid enzymatic method for the microdetermination of serum free fatty acids showed a close correlation with the chemical colorimetric method.
Abstract
A reliable, simple, and rapid enzymatic method is described for the microdetermination of serum free fatty acids. The principle of the method is based on the activation of free fatty acids by a bacterial acyl-CoA synthetase (EC 6.2.1.3). The reaction is followed as production of AMP using the myokinase-pyruvate kinase-lactate dehydrogenase system as an indicator reaction. Results on the determination using human and rabbit sera showed a close correlation with the chemical colorimetric method.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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