Termination of transcription in the mouse α-amylase gene Amy-2a occurs at multiple sites downstream of the polyadenylation site
CellPublished 1 October 1984
Otto Hagenbüchle, Peter K. Wellauer, David L. Cribbs, Ueli Schibler
Citations130
SJR quartileQ1
SJR score22.61
SNIP7.62
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TL;DR
Mapping of in vitro elongated nascent transcripts to Amy-2a restriction fragments indicates that transcription terminates in a region between 2.5 and 4 kb downstream of the polyadenylation site, suggesting that transcription termination occurs at multiple sites.
Abstract
We have delimited the region of transcription termination in the alpha-amylase gene Amy-2a. Mapping of in vitro elongated nascent transcripts to Amy-2a restriction fragments indicates that transcription terminates in a region between 2.5 and 4 kb downstream of the polyadenylation site. These runoff transcription experiments, combined with S1 nuclease mapping of nuclear transcripts at steady state, suggest that transcription termination occurs at multiple sites.
Keywords
Biochemistry, Genetics and Molecular Biology
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