A sensitive method for the assay of hyaluronidase activity
Generate an AI Snapshot to get a quick, structured summary of this paper.
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
A spectrophotometric method based on the binding of a carbocyanine dye to undegraded substrate results in a spectral shift with an absorbance maximum at 640 nm which is proportional to the amount of hyaluronic acid within a prescribed range.
Abstract
A spectrophotometric method for the assay of hyaluronidase activity, based on the binding of a carbocyanine dye (1-ethyl-2-[3-(1-ethyl-naphtho[1,2d]thiazolin-2-ylidene)-2-methyl-propenyl]naphtho[1,2d]thiazolium bromide) to undegraded substrate, is described. The binding results in a spectral shift with an absorbance maximum at 640 nm which is proportional to the amount of hyaluronic acid within a prescribed range. The end products of hyaluronidase activity do not cause a shift in the dye spectrum. The method allows the determination of an amount of hyaluronidase equivalent to 0.00005 NF unit.