Tracer methodology in whole body and organ balance metabolic studies: plasma sampling is required. A study in post-absorptive rats using isotopically labeled arginine, phenylalanine, valine and leucine
Clinical NutritionPublished 1 June 2000
M.M. Hallemeesch, Peter B. Soeters, Nicolaas E.P. Deutz
Citations17
SJR quartileQ1
SJR score2.21
SNIP2.02
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TL;DR
Plasma sampling is recommended and whole blood sampling is strongly advised against in metabolic organ balance studies in which amino acid tracers are used, because of overestimations of whole body and organ production rates.
Abstract
From our data we recommend plasma sampling and strongly advise against whole blood sampling in metabolic organ balance studies in which amino acid tracers are used.
Keywords
Biochemistry, Genetics and Molecular Biology
Experimental and surgical technique in the rat
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