A glossary for the social epidemiology of work organisation: Part 3, Terms from the sociology of labour markets

This is part 3 of a three-part glossary on the social epidemiology of work organisation, which presents concepts related to labour markets drawn from economics, business and sociology and to contemporary ideas on post-industrialisation and globalisation.

Abstract

Summary Bacterial type II secretion systems (T2SS) translocate virulence factors, toxins and enzymes across the cell outer membrane (OM). An assembled T2SS has not yet been isolated in vitro. Here we use a fusion of negative stain and cryo-electron microscopy (EM) to reveal the core architecture of an assembled T2SS from the pathogen Klebsiella pneumoniae. We show that 7 proteins form a ∼2.5 MDa complex that spans the cell envelope. The outer membrane complex (OMC) includes the secretin PulD with all domains modelled and the pilotin PulS. The inner membrane assembly platform (AP) components have an overall stoichiometry of six copies each. The AP includes PulE and the cytoplasmic domain of PulL, which combine to form a flexible hexameric hub consistent with an inactive state. Symmetry mismatch between the OMC and AP is overcome by PulC linkers spanning the periplasm with PulC HR domains binding independently at the secretin base. Our results show the T2SS to have a highly dynamic modular architecture with implication for pseudo-pilus assembly and substrate loading.