Measuring Intra-Assay Agreement for the Ames Salmonella Assay
Lecture notes in medical informaticsPublished 1 January 1991
Walter W. Piegorsch, Errol Zeiger
Citations83
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TL;DR
The Salmonella assay occupies a central role in the genotoxicity testing program undertaken by the NTP, and has involved numerous chemicals, tested under code, and under strictly controlled protocols at a number of laboratories.
Abstract
To assess the effects of exposure to toxic stimuli, the U.S. National Toxicology Program (NTP) conducts bioassays in various organisms, each with a different biological endpoint. Mutagenic damage, as indicated by the Salmonella assay (Ames et al., 1975), is a major concern. The Salmonella assay occupies a central role in the genotoxicity testing program undertaken by the NTP. This program has involved numerous chemicals, tested under code, and under strictly controlled protocols at a number of laboratories.
Keywords
Biochemistry, Genetics and Molecular BiologyChemical Engineering
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The preincubation modification of the Salmonella/mammalian microsome assay was used to test chemicals in up to fiveSalmonella strains in the presence and absence of rat and hamster liver S-9.
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The tests were conducted using a preincubation protocol in the absence of exogenous metabolic activation, and in the presence of liver S‐9 from Aroclor‐induced male Sprague‐Dawley rats and Syrian hamsters, to establish mutagenicity in Salmonella typhimurium.
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All chemicals were tested under code using a preincubation modification of the Salmonella/microsome test in the absence of exogenous metabolic activation and in the presence of liver S-9 from Aroclor-induced male Sprague-Dawley rats and Syrian hamsters.
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Three hundred chemicals were tested for mutagenicity, under code, in Salmonella typhimurium, using a preincubation protocol, and the results and data from these tests are presented.
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196 Citations1985Virginia C. Dunkel, Errol Zeiger +6 more
The intra- and interlaboratory reproducibility of the Salmonella assay with regard to the overall judgment of mutagenic or nonmutagenic was good, but the results in the E coli strain exhibited a high degree of variability between laboratories.
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80 Citations1986Shulamith T. Gross
A modified kappa coefficient of agreement for multiple categories is proposed and a parameter-free distribution for testing null agreement is provided, for use when the number of raters is large relative to the numberof categories and subjects.
BiometricsOn Assessing Interrater Agreement for Multiple Attribute Responses
51 Citations1989Lawrence L. Kupper, Kerry B. Hafner
A two-rater concordance statistic is derived, and associated statistical inference-making procedures are provided for assessing the extent of interrater agreement when each unit to be rated is characterized by a (possibly empty) subset of a specified set of distinct nominal attributes.