Functional role for c-myc in mitogenic response to platelet-derived growth factor
NaturePublished 1 August 1984
Hugo A. Armelin, M C S Armelin, Kathleen Kelly, Timothy A. Stewart, Philip Leder, Brent Cochran
Citations578
SJR quartileQ1
SJR score18.29
SNIP10.16
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TL;DR
Both the human and the mouse c-myc genes stimulate clonal growth of 3T3 cells in PDGF-free medium suggesting new strategies for analysis of oncogenes which do not function in focus formation assays.
Abstract
In BALB/c-3T3 cells, expression of the c-myc gene is stimulated by platelet-derived growth factor (PDGF). Using mouse mammary tumour virus promoter: c-myc recombinant plasmids, 3T3 sublines were constructed in which hydrocortisone was the primary determinant of myc mRNA content. The c-myc gene product is an intracellular mediator of the growth response to PDGF though probably not the only one. Both the human and the mouse c-myc genes stimulate clonal growth of 3T3 cells in PDGF-free medium suggesting new strategies for analysis of oncogenes which do not function in focus formation assays.
Keywords
Immunology and MicrobiologyMedicineBiochemistry, Genetics and Molecular Biology
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