Polyribosome dissociation and formation in intact reticulocytes with conservation of messenger ribonucleic acid.
Proceedings of the National Academy of SciencesPublished 1 June 1965Open access
P A Marks, Edward R. Burka, F. Conconi, William Perl, R A Rifkind
Citations91
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
Evidence is presented that during incubation of reticulocytes in the presence of NaF, polyribosome dissociation to 80S ribosomes and ribosomal subunits proceeds by simple first-order kinetics for polyribsomes of all sizes, suggesting that this process is not a reversal of the process of dissociation.
Abstract
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
Keywords
Biochemistry, Genetics and Molecular Biology
Proceedings of the National Academy of SciencesA MULTIPLE RIBOSOMAL STRUCTURE IN PROTEIN SYNTHESIS
586 Citations1963Jonathan R. Warner, Paul M. Knopf +1 more
Proceedings of the National Academy of SciencesPOLYRIBOSOMES IN NORMAL AND POLIOVIRUS-INFECTED HELA CELLS AND THEIR RELATIONSHIP TO MESSENGER-RNA
469 Citations1963Sheldon Penman, Klaus Scherrer +2 more
The brief appearance of thymidine kinase activity prior to DNA synthesis in microspores of Lilium is due to a de novo synthesis of protein.
Journal of Molecular BiologyFunction of aggregated reticulocyte ribosomes in protein synthesis
342 Citations1963Alfred Gierer
The results indicate that many of the aggregated ribosomes contain messenger RNA, but are not receptive to added messenger RNA; whereas many ofThe unaggregated ribsomes do not contain messengerRNA,but are receptive toadded messenger RNA molecules.
Journal of Molecular BiologyPolypeptide synthesis in Escherichia coli
215 Citations1963Walter Gilbert
The poly U-directed† synthesis of polyphenylalanine in the cell-free system from Escherichia coli is used as a model system in which to investigate the interaction of messenger RNA with ribosomes and it is shown that both the 50 s and the 30 s ribosome are necessary for polypeptide synthesis.
Proceedings of the National Academy of SciencesPROTEIN SYNTHESIS IN ERYTHROID CELLS, I. RETICULOCYTE RIBOSOMES ACTIVE IN STIMULATING AMINO ACID INCORPORATION
185 Citations1962Paul A. Marks, Edward R. Burka +1 more
Journal of Molecular BiologyProtein synthesis by polyribosomes on protoplast membranes of B. megaterium
129 Citations1963David Schlessinger
The sum of the evidence supports the current hypothesis that protein synthesis in bacteria occurs on polyribosomes, each composed of a number of ribosomes serviced by a molecule of M-RNA; the functioning complexes may be stabilized on the cell membrane.
Journal of Clinical InvestigationSUBSTANCES STIMULATING GLUCOSE CATABOLISM BY THE OXIDATIVE REACTIONS OF THE PENTOSE PHOSPHATE PATHWAY IN HUMAN ERYTHROCYTES*
121 Citations1961À. Szeinberg, Paul A. Marks
A study has been made of the effect of the physiological substances-cysteine, ascorbic acid and pyruvate-and of agents which can cause hemolytic anemia-acetyl-phenylhydrazine, nitrofurantoin, cysteine and fava to produce a relative insuffi-ciency in the supply of TPNH.
Journal of Molecular BiologyThe visualization of polyribosomal structure
115 Citations1963Henry S. Slayter, Jonathan R. Warner +2 more
Polyribosomes from rabbit reticulocytes have been studied in the electron microscope using positive- and negative-staining techniques and the relation between the polyribosome and the length of the messenger RNA for hemoglobin is discussed.
The Journal of Cell BiologyALTERATIONS IN POLYRIBOSOMES DURING ERYTHROID CELL MATURATION
111 Citations1964Richard A. Rifkind, D. Danon +1 more
A morphological study of the changes in ribosome content and organization which occur during the maturation of erythroid cells of the phenylhydrazine-treated rabbit and possible models which may account for the stability of the polyribosome and for the mechanism of polyribsome dissociation are discussed.
Archives of Biochemistry and BiophysicsFormation of active ribosomal aggregates (polysomes) upon fertilization and development of sea urchin eggs
101 Citations1963Alberto Monroy, Albert Tyler
Ribosomes from unfertilized eggs and developmental stages of Paracentrotus lividus and Lytechinus pictus have been fractionated in sucrose gradients and Pulse-labeling experiments support their identification as the active particles in amino acid incorporation.
Proceedings of the National Academy of SciencesPOLYRIBOSOMES AND PROTEIN SYNTHESIS DURING RETICULOCYTE MATURATION IN VITRO
95 Citations1963Paul A. Marks, Richard A. Rifkind +1 more
Reticulocytes were prepared from phenylhydrazine-treated rabbits according to the procedures of Borsook et al. using a siliconized, water-jacketed incubation flask fitted with a cap and maintained constant at 7.37 by means of a pH-Stat.
Journal of Molecular BiologyOccurrence and function of polysomes in rabbit reticulocytes
93 Citations1964A. P. Mathias, R. Williamson +2 more
The ribosomes of the polysome are linked by a substance sensitive to very low concentrations of pancreatic ribonuclease, presumably an RNA molecule.
Journal of Molecular BiologyPolyribosomes and the loss of hemoglobin synthesis in the maturing reticulocyte
79 Citations1965Ellen R. Glowacki, Robert L. Millette
The fate of the protein-synthesizing system in rabbit reticulocytes as they mature to erythrocytes has been examined and evidence indicates that the process of maturation involves primarily the loss of total ribosomal material rather than a sequential breakdown of polyribosomes to 80 S particles.
Developmental BiologyOn the mechanism of ribosomal activation in newly fertilized sea urchin eggs
51 Citations1964T. Hultin
The formation of active ribosomal aggregates in the newly fertilized eggs is a strictly energy-requiring process, initiated by the cortical reaction, and presumably under the influence of messenger RNA.
Proceedings of the National Academy of SciencesPOLYRIBOSOME FORMATION AND HEMOGLOBIN SYNTHESIS
31 Citations1963Britta Denise Hardesty, John J. Hutton +2 more
The sample was taken through the complete washing procedure and the per cent of N-terminal C'4-valine in hemoglobin was determined by the dinitrofluorobenzene method of Bishop et al.
Proceedings of the National Academy of SciencesSIZE OF POLYRIBOSOMES IN INTACT RETICULOCYTES
23 Citations1964Richard A. Rifkind, Lucio Luzzatto +1 more
Examining in greater detail the ultrastructure of the filament-polyribosome complexes, evidence is presented that reticulocytes contain polyribosomes containing as many as 20 individual ribosomes.
