Membrane fusion of mumps virus with ghost erythrocytes and CV-1 cells
VirologyPublished 1 November 1992
Christopher Di Simone, John D. Baldeschwieler
Citations8
SJR quartileQ2
SJR score0.74
SNIP0.60
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TL;DR
The octadecyl rhodamine (R18) fluorescent dequenching assay was used to examine membrane fusion between mumps virus and mammalian cells and Fetuin was found to inhibit virus fusion, suggesting a role for sialic acid in virus binding to the cells.
Abstract
The octadecyl rhodamine (R18) fluorescent dequenching assay was used to examine membrane fusion between mumps virus and mammalian cells. Rapid fluorescent dequenching, indicative of membrane fusion, was observed when labeled mumps virus was mixed with either ghost erythrocytes or CV-1 cells. After 15 min a saturation limit of 18 virus per erythrocyte ghost and 6400 virus per CV-1 cell was observed. Fetuin was found to inhibit virus fusion, suggesting a role for sialic acid in virus binding to the cells. Two dequenching processes were observed of which the faster process is thought to be membrane fusion and the second process is thought to be probe proximal transfer.
Keywords
Immunology and MicrobiologyMedicineBiochemistry, Genetics and Molecular Biology
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