A kinetic estimation of base sequence complexity of nuclear poly(A)-containing RNA in mouse friend cells

Complementary DNA (cDNA) has been transcribed by viral reverse transcriptase from poly(A)-containing nuclear RNA prepared from growing mouse Friend cells (clone M2), and kinetics of hybridization of cDNA to template RNA indicate that nuclear poly( A)-containing RNA consists of at least two abundance classes.

Abstract

Complementary DNA (cDNA) has been transcribed by viral reverse transcriptase from poly(A)-containing nuclear RNA prepared from growing mouse Friend cells (clone M2). Annealing experiments with this cDNA have demonstrated that a large proportion of the poly(A) tracts in M2 cell nuclear RNA are adjacent to RNA sequences which are transcribed from nonrepetitive DNA in the mouse genome. The kinetics of hybridization of cDNA to template RNA indicate that nuclear poly(A)-containing RNA consists of at least two abundance classes, the more complex of which is transcribed from approximately 3 percent of the genome. Thus there are at least 5 times more unique DNA sequences represented in nuclear polyadenylated RNA than in polysomal polyadenylated RNA. Moreover, there is evidence for posttranscriptional mechanisms which alter the relative concentrations of some (at least) gene transcripts between nucleus and cytoplasm.