The mechanism of vesicle formation
Biochemical JournalPublished 15 November 1988Open access
D. D. Lasič
Citations489
SJR quartileQ1
SJR score2.06
SNIP0.98
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
Vesicles are very important in many different areas of science and technology and serve as delivery vehicles for drugs, genetic material, enzymes and other (macro)molecules into living cells and through other hydrophobic barriers in pharmacology, medicine, genetic engineering, cosmetic industry and food industry.
Keywords
ChemistryBiochemistry, Genetics and Molecular Biology
Zeitschrift für Naturforschung CElastic Properties of Lipid Bilayers: Theory and Possible Experiments
6,204 Citations1973W. Helfrich
A theory of the elasticity of lipid bilayers is proposed and it is argued that in the case of vesicles (= closed bilayer films) the only elasticity controlling nonspherical shapes is that of curvature.
Journal of Molecular BiologyDiffusion of univalent ions across the lamellae of swollen phospholipids
4,554 Citations1965A. D. Bangham, M. M. Standish +1 more
It is found that as the surface charge of the lipid lamellae is increased, the amount of cation per μmle of lipid increases, and the phospholipid liquid crystalline structures appear to “bind” or “capture” cations.
Proceedings of the National Academy of SciencesProcedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation.
2,303 Citations1978F Szoka, Demetrios Papahadjopoulos
A substantial fraction of the aqueous phase is entrapped within the vesicles, encapsulating even large macromolecular assemblies with high efficiency, and has unique advantages for encapsulating valuable water-soluble materials such as drugs, proteins, nucleic acids, and other biochemical reagents.
Journal of Molecular BiologyNegative staining of phospholipids and their structural modification by surface-active agents as observed in the electron microscope
1,760 Citations1964A. D. Bangham, R.W. Horne
The negative-staining technique was applied to the study of lipid phase structures in the electron microscope and lecithin dispersions were shown to be progressively disrupted after mixing with lysolecithin in water.
BiochemistryPhosphatidylcholine vesicles. Formation and physical characteristics
1,525 Citations1969Ching-hsien Huang
Annual Review of Biophysics and BioengineeringComparative Properties and Methods of Preparation of Lipid Vesicles (Liposomes)
1,504 Citations1980F Szoka, D. Papahadjopoulos
This research attacked the mode of action of phosphatidylcholine-like deposits in response to the presence of ribonucleic acid by exploiting its role as a “spatially aggregating substance” in the response to EMT.
Quarterly Reviews of BiophysicsPhysical principles of membrane organization
1,390 Citations1980Jacob N. Israelachvili, S. Marĉelja +1 more
An understanding of the physical principles that govern the molecular organization of membranes is essential for an understanding of their physiological roles since structure and function are much more interdependent in membranes than in, say, simple chemical reactions in solution.
Biochimica et Biophysica Acta (BBA) - BiomembranesSingle bilayer liposomes prepared without sonication
1,335 Citations1973Shmuel Batzri, Edward D. Korn
Single bilayer liposomes, indistinguishable from those obtained by sonication, can be prepared by injecting an thanolic solution of phospholipid into water by injection of dilute suspension.
Biochimica et Biophysica Acta (BBA) - BiomembranesPreparation of liposomes of defined size distribution by extrusion through polycarbonate membranes
1,101 Citations1979Fred C. Olson, C. Anthony Hunt +3 more
Liposomes of defined size and homogeneity have been prepared by sequential extrusion of the usual multilamellar vesicles through polycarbonate membranes, which can double the encapsulation efficiency of the liposome preparation.
Biochemical JournalStabilization of dry phospholipid bilayers and proteins by sugars
923 Citations1987J.H. Crowe, Lois M. Crowe +2 more
Biochimica et Biophysica Acta (BBA) - BiomembranesLarge volume liposomes by an ether vaporization method
514 Citations1976David W. Deamer, A. D. Bangham
Liposomes, in the size range of microsomal vesicles, are formed when ether solutions of a variety of lipids are injected into warm aqueous solutions and the volume trapping efficiency is approximately ten times that of sonicated and hand-shaken preparations.
BiochemistryQuasielastic light-scattering studies of aqueous biliary lipid systems. Mixed micelle formation in bile salt-lecithin solutions
510 Citations1980Norman A. Mazer, George B. Benedek +1 more
The inclusion of bile salts in a fixed stoichiometry within the interior of the bilayers is shown to provide a quantitative explanation for the divergence of the mixed micellar sizes, their temperature dependence, and the origin of the lecithin-bile salt phase limit.
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid MetabolismStudies on simple and mixed bile salt micelles by nuclear magnetic resonance spectroscopy
384 Citations1969Donald Small, S. A. Penkett +1 more
High resolution nuclear magnetic resonance (NMR) spectroscopy has been used to study the spectra of the common bile acids in organic solvents, the structure of the bile-salt micelle and the interaction of bile salt with lecithin in mixed micelles, consistent with the hypothesis that the leCithin-bile salt micelle exists as a bilayer of lecitin surrounded on its hydrophobic surfaces by bile salts.
Biochimica et Biophysica Acta (BBA) - BiomembranesSingle bilayer vesicles prepared without sonication physico-chemical properties
383 Citations1976Josef Brunner, Peter Skrabal +1 more
A homogeneous population of pure lecithin single-bilayer vesicles free of multilamellar structures is obtained, and the vesicle diameter is somewhat larger than the average diameter of sonication.
Biochimica et Biophysica Acta (BBA) - BiomembranesPhospholipid model membranes. II. Permeability properties of hydrated liquid crystals
357 Citations1967Demetrios Papahadjopoulos, J.C. Watkins
The rates of diffusional exchange of trapped ions through hydrated liquid crystals and related vesicular structures composed of various naturally occurring, purified phospholipids were determined and it was concluded that this system constitutes a valid model for many biological membrane phenomena.
Archives of Biochemistry and BiophysicsPreservation of freeze-dried liposomes by trehalose
346 Citations1985Lois M. Crowe, John H. Crowe +3 more
Evidence is provided that stabilization of the dry liposomes requires depression of transition temperature and consequent maintenance of the constituent lipids in the dryliposomes in a liquid crystalline phase.
Physical Review LettersUnbinding Transitions of Interacting Membranes
338 Citations1986Reinhard Lipowsky, Stanislas Leibler
A systematic theory of the interplay among these interactions reveals a continuous phase transition from a state where the membranes are bound together to a stateWhere they are completely separated, in three dimensions, driven by fluctuations.
Proceedings of the National Academy of SciencesStabilization of lipid bilayer vesicles by sucrose during freezing
244 Citations1986George Strauss, H. Häuser
It is concluded that the extent of membrane disruption depends on the membrane mobility at the moment of freezing and that sucrose exerts its protective effect by binding to the membrane interface and/or by affecting the water structure.
Journal of Pharmacy and PharmacologyUltrasonic Irradiation of Some Phospholipid Sols
186 Citations1962L. Saunders, J.H. Perrin +1 more
The size and shape of micelles in the irradiated sols has been studied by means of viscosity and diffusion measurements and by determination of sedimentation coefficients in the ultra‐centrifuge.
BiochemistryKinetics and structural aspects of reconstitution of phosphatidylcholine vesicles by dilution of phosphatidylcholine-sodium cholate mixed micelles
142 Citations1986Shlomo Almog, Tamar Kushnir +2 more
The main findings and conclusions are the following: Both the state of aggregation and the apparent equilibrium size distribution of micelles or vesicles obtained by dilution of the PC-cholate mixed micellar dispersions are a function of the cholate to PC molar ratio in the mixed aggregates.
BiochemistrySpontaneous formation of stable unilamellar vesicles
141 Citations1984Nathan Gabriel, Mary F. Roberts
Stable unilamellar vesicles form spontaneously upon mixing aqueous suspensions of long-chain lecithins (fatty acid chain lengths 14 carbons or longer) with small amounts (20 mol %) of micellar synthetic short-chainLecithin (Fatty acid Chain) lengths 6-8 carbons.
ScienceSpontaneous Vesicles Formed from Hydroxide Surfactants: Evidence from Electron Microscopy
130 Citations1983Yeshayahu Talmon, D. F. Evans +1 more
Dialkyldimethylammonium hydroxide surfactants are highly soluble in water and form spontaneous stable vesicles, and appear to provide an ideal membrane mimetic system for the study of fusion and ion transport.
Biochimica et Biophysica Acta (BBA) - BiomembranesA molecular model for vesicle formation
104 Citations1982Danilo D. Lasiç
A molecular model based on a balance between elastic and ‘hydrophobic’ forces for various micelle and bilayer geometries in dilute aqueous solutions predicts bilayered disc-like transition structures and is in agreement with experimental data.
Pharmacy World & SciencePreparation of liposomes via detergent removal from mixed micelles by dilution
93 Citations1986Wim Jiskoot, Tom Teerlink +2 more
Fairly homogeneous liposomes with mean diameters ranging from 100 to over 200 nm could be obtained, and the particle size was dependent on cholesterol content and surface charge, and could be reproducibly controlled by adjustment of the dilution rate.
BiochemistryMultilayered vesicles prepared by reverse-phase evaporation: liposome structure and optimum solute entrapment
85 Citations1987Charles Pidgeon, Susan McNeely +2 more
X-ray diffraction data demonstrate that a heterogeneous distribution of lipid may exist in multilayered vesicles prepared by the REV process and a method to calculate the fraction of water residing in the liposomes' core is presented and used to compare multilayer vesicle prepared by different processes.
ScienceSize Analysis of Phospholipid Vesicle Preparations
79 Citations1982Yasuhiko Nozaki, Danilo D. Lasiç +2 more
Gel exclusion chromatography based on the use of Sephacryl S-1000 provides a quick and convenient method for determining the average diameter of phospholipid vesicles and an approximate measure of size heterogeneity.
Journal of the American Oil Chemists SocietyLiquid crystals and living systems
79 Citations1968James L. Fergason, Glenn H. Brown
Of particular interest in living systems are liquid crystals, which are formed by cholesteric esters and many protein materials, and the two-dimensional crystals (smectic structure), which are form by fatty acid derivatives.
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid MetabolismThe nature and origin of the NMR spectrum of unsonicated and sonicated aqueous egg yolk lecithin dispersions
78 Citations1972E.G. Finer, A.G. Flook +1 more
Calculations of expected line narrowing as a function of the radius of a particle tumbling in water showed that the observation after sonication of a high resolution spectrum, with the different linewidths observed, can be explained simply by the extent to which tumbling of small particles averages out dipolar line broadening.
Biochimica et Biophysica Acta (BBA) - Reviews on Bioenergeticsmembrane reconstitution of the enrgy-conserving enzymes of oxidative phosphorylation
67 Citations1984Robert P. Casey
Proceedings of the National Academy of SciencesSpontaneous vesiculation of phospholipids: a simple and quick method of forming unilamellar vesicles.
63 Citations1982H. Häuser, Nigel Gains
A good correlation was found between the proportion of phospholipid giving rise to the high resolution spectrum, asderived from spectral intensity measurements, and the proportion present as small unilamellar vesicles, as derived from gel filtration.
Biochimica et Biophysica Acta (BBA) - BiomembranesPreparation of monodisperse vesicles with variable size by dilution of mixed micellar solutions of bile salt and phosphatidylcholine
63 Citations1984Peter Schurtenberger, Norman A. Mazer +2 more
The combination of dilution and dialysis represents a method for the preparation of unilamellar, monodisperse and detergent-free vesicles with a desired radius that can be chosen between 120 and 500 A.
Biochemical JournalDynamics of proteoliposome formation. Intermediate states during detergent dialysis
42 Citations1987John M. Wrigglesworth, M S Wooster +2 more
The results indicate that proteoliposome formation by the detergent-dialysis procedure takes place in three distinct stages, and the orientation of membrane protein in the final vesicles appears to be determined by the effect of protein conformation on the initial curvature of the bilayer sheets during the fragmentation process.
Biochimica et Biophysica Acta (BBA) - BiomembranesPreparation of multilamellar vesicles of defined size-distribution by solvent-spherule evaporation
37 Citations1985Sinil Kim, Russell E. Jacobs +1 more
This is the first fundamentally new method of multilamellar vesicle preparation since Bangham's report in 1965 and can be adjusted by varying the duration of mechanical agitation of the spherules and the concentration of amphipathic lipids in the solvents.
Chemistry and Physics of LipidsSpontaneous vesiculation of uncharged phospholipid dispersions consisting of lecithin and lysolecithin
37 Citations1987H. Häuser
The work presented here demonstrates that the phenomenon of spontaneous vesiculation is not restricted to charged lipids and lipid mixtures, but occurs also in isoelectric phospholipid mixtures consisting of egg EPC and egg lysophosphatidylcholine (lyso-EPC), using 1H high-resolution NMR and freeze-fracture electron microscopy to characterize the mixed EPC/lyso EPC dispersions in excess H2O.
Biochimica et Biophysica Acta (BBA) - BiomembranesModel transport studies utilizing lecithin spherules
24 Citations1972Zaka-ud-din T. Chowhan, Toshihisa Yotsuyanagi +1 more
A systematic evaluation of all the parameters which could introduce uncertainties revealed that the monosize-multiconcentric models are generally in satisfactory agreement with the experimental transport data, and suggest that these models may be used in the reliable determinations of effective bulk permeability coefficients.
Biochimica et Biophysica Acta (BBA) - BiomembranesStudies of asymmetric membrane assembly
11 Citations1977Craig Zwizinski, William Wickner
The major capsid protein of M13 bacteriophage is incorporated at each stage of infection into the host plasma membrane with its amino terminus exposed on the outer surface and a model for this assembly reaction is proposed.