Characterization of the central region containing the X-inactivation center and terminal region of the mouse X Chromosome using irradiation and fusion gene transfer hybrids
Mammalian GenomePublished 1 January 1991
Louise Sefton, Danielle Arnaud, Peter N. Goodfellow, Marie‐Christine Simmler, Philip Avner
Citations15
SJR quartileQ2
SJR score0.98
SNIP0.75
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TL;DR
Two large panels of somatic cell hybrids that contain mouse X Chromosome (Chr) fragments have been generated by irradiating the monochromosomal mouse-hamster hybrid HYBX, which retains the mouse X Chr, followed by fusion with a recipient Chinese hamster cell line.
Abstract
The irradiation and fusion gene transfer (IFGT) procedure provides a means of isolating subchromosomal fragments for use in the mapping of loci and for cloning probes from a particular area of a chromosome. Using this procedure, two large panels of somatic cell hybrids that contain mouse X Chromosome (Chr) fragments have been generated. These hybrid panels were generated by irradiating the monochromosomal mouse-hamster hybrid HYBX, which retains the mouse X Chr, with either 10 K or 50 K rads of X-irradiation followed by fusion with a recipient Chinese hamster cell line. IFGT hybrids retaining mouse material were generated at high frequency. These hybrids were used to orient loci in the X-inactivation center region that had not been resolvable in our interspecies backcross panel and also to map, within the terminal region of the X Chr, repeat elements detected by the probe p15-4. These hybrids not only complement existing interspecies meiotic mapping panels for the detailed analysis of specific regions of particular chromosomes, but also provide a potential source of material for chromosome-specific probe isolation.
Keywords
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