Chapter 7 Methods for Monitoring the Growth of Yeast Cultures and for Dealing with the Clumping Problem
Methods in cell biologyPublished 1 January 1975
John R. Pringle, Juan‐R. Mor
Citations98
SJR quartileQ4
SJR score0.30
SNIP0.22
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TL;DR
This chapter describes the clumping problem, methods for measuring biomass and its components and cytokinesis, and the virtues and limitations of (and the relationships among) various methods of monitoring growth.
Abstract
This chapter discusses methods for monitoring the growth of yeast cultures. Most biochemical, physiological, cytological, and developmental studies of yeasts require monitoring the growth of yeast cultures. In some cases, determination of the rate or extent of growth provides important experimental results; in many other cases, it is necessary to know the amount of cellular material analyzed or the physiological state of the cells, or both. The methods used for determining amounts of yeast and for monitoring the growth of yeast cultures are the same as those used with other microorganisms. This chapter focuses on the virtues and limitations of (and the relationships among) various methods of monitoring growth. It discusses the complications imposed by the budding mode of reproduction and by the clumping problem. The chapter describes the clumping problem, methods for measuring biomass and its components and cytokinesis.
Keywords
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