Expression of the liver-enriched transcriptional activator protein DBP follows a stringent circadian rhythm
CellPublished 1 December 1990
Jérôme Wuarin, Ueli Schibler
Citations218
SJR quartileQ1
SJR score22.61
SNIP7.62
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
The liver-enriched transcriptional activator protein DBP accumulates in hepatocytes of adult rats according to a strictly controlled circadian rhythm, and the albumin gene, a putative target of DBP, is transcribed more efficiently in the evening than in the morning.
Abstract
The liver-enriched transcriptional activator protein DBP accumulates in hepatocytes of adult rats according to a strictly controlled circadian rhythm. DBP is not detectable in liver nuclei during the morning hours. Its level raises sharply during the afternoon and reaches a maximum at about 8 p.m. During the night the cellular DBP concentration decreases below detectability. This oscillation is "free running," transcriptionally regulated, and may be under the negative control of glucocorticoid hormones. In keeping with the rhythmicity of DBP accumulation, the albumin gene, a putative target of DBP, is transcribed more efficiently in the evening than in the morning.
Keywords
MedicineNeuroscienceBiochemistry, Genetics and Molecular Biology
NatureCleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
251,640 Citations1970Ulrich K. Laemmli
Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Molecular Cloning: A Laboratory Manual 2
129,982 Citations1989Joseph Sambrook, Elisabeth Fritsch +1 more
The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories.
Biomedicine & PharmacotherapyAntibodies, a laboratory manual
12,466 Citations1989
A second edition of Antibodies: A Laboratory Manual is being published in September 2013, Revised, extended and updated by Edward Greenfield of the Dana-Farber Cancer Center, the material has been recast with extensive new information and new chapters have been added.
NatureHow eukaryotic transcriptional activators work
1,896 Citations1988Mark Ptashne
A specific protein, bound to DNA, can activate transcription of a wide array of genes in many eukaryotes and is controlled by the immune system.
ScienceTransplanted Suprachiasmatic Nucleus Determines Circadian Period
1,832 Citations1990Martin R. Ralph, F. Foster +2 more
The pacemaker role of the suprachiasmatic nucleus in a mammalian circadian system was tested by neural transplantation by using a mutant strain of hamster that shows a short circadian period to restore circadian rhythms to arrhythmic animals whose own nucleus had been ablated.
ScienceRegulation of Inducible and Tissue-Specific Gene Expression
1,642 Citations1987Tom Maniatis, Stephen Goodbourn +1 more
A number of cases of inducible and tissue-specific gene expression involve the activation of preexisting transcription factors, rather than the synthesis of new proteins, which may involve covalent modification of the protein or an allosteric change in its structure.
CellTissue-specific in vitro transcription from the mouse albumin promoter
1,316 Citations1986Kathryn Gorski, M. Carneiro +1 more
Transcriptionally active nuclear extracts have been prepared from rat liver, brain, and spleen and the adenovirus-2 major late promoter directs efficient transcription by RNA polymerase II in all of these extracts, whereas the promoter of the mouse albumin gene is significantly used only in the liver extract.
NatureFeedback of the Drosophila period gene product on circadian cycling of its messenger RNA levels
1,157 Citations1990Paul E. Hardin, Jeffrey C. Hall +1 more
Observations indicate that the cycling of per-encoded protein could result from per RNA cycling, and that there is a feedback loop through which the activity of each per gene product causes cycling of its own RNA.
CellA tissue-specific transcription factor containing a homeodomain specifies a pituitary phenotype
1,002 Citations1988Holly A. Ingraham, Ruoping Chen +7 more
The structure of Pit-1 and its recognition elements suggests that metazoan tissue phenotype is controlled by a family of transcription factors that bind to related cis-active elements and contain several highly conserved domains.
CellThe pituitary-specific transcription factor GHF-1 is a homeobox-containing protein
771 Citations1988Mordechai Bodner, José-Luis Castriilo +4 more
The isolation of bovine and rat GHF-1 cDNA clones is described and it is indicated that this region of the protein functions as its DNA binding domain, which correlates with the selective transcription of its target, the GH gene.
ScienceA Mutation of the Circadian System in Golden Hamsters
696 Citations1988Martin R. Ralph, Michael Menaker
CellThe gradient morphogen bicoid is a concentration-dependent transcriptional activator
670 Citations1989Gary Struhl, Kevin Struhl +1 more
It is suggested that the bcd gradient has the instructive capacity to activate other subordinate control genes by the same mechanism, each in a distinct spatial domain according to its affinity for bcd protein.
ScienceThe DNA Binding Domain of the Rat Liver Nuclear Protein C/EBP Is Bipartite
660 Citations1989William Landschulz, Peter F. Johnson +1 more
Experimental tests now provide support for the leucine zipper hypothesis, which predicts a new structural motif that plays a role in facilitating sequence-specific interaction between protein and DNA.
Genes & DevelopmentTissue-specific expression, developmental regulation, and genetic mapping of the gene encoding CCAAT/enhancer binding protein.
637 Citations1989E H Birkenmeier, Babette Gwynn +5 more
Observations raise the possibility that the synthesis of C/EBP may be responsive to humoral factors and that modulation in C/ EBP expression might mediate coordinated changes in gene expression that facilitate adaptive challenges met during development or during the fluctuating physiological states of adult life.
Genes & DevelopmentLAP, a novel member of the C/EBP gene family, encodes a liver-enriched transcriptional activator protein.
595 Citations1990Patrick Descombes, Mario Chojkier +3 more
Although correctly initiated transcripts from the LAP gene accumulate in the six examined tissues--liver, lung, spleen, kidney, brain, and testis--LAP protein is highly enriched in liver nuclei, the preferential accumulation of LAP protein in liver appears to be regulated post-transcriptionally.
CellThe interplay of DNA-binding proteins on the promoter of the mouse albumin gene
535 Citations1987Serge Lichtsteiner, Jérôme Wuarin +1 more
The promoter of the mouse albumin gene contains at least six binding sites for specific DNA-binding proteins (A to F), and both of these competing binding sites are required for maximal in vitro transcription.
NatureA cloned octamer transcription factor stimulates transcription from lymphoid–specific promoters in non–B cells
493 Citations1988Michael M. Müller, Siegfried Ruppert +2 more
The complementary DNA coding for a lymphocyte-specific transcription factor binding to the DNA 'octamer' sequence TNATTTGCAT has been cloned and the nucleotide sequence shows homology to the homoeobox domain.
Genes & DevelopmentCCAAT/enhancer binding protein activates the promoter of the serum albumin gene in cultured hepatoma cells.
484 Citations1989Alan D. Friedman, William Landschulz +1 more
It is speculated that C/EBP may play a general role in establishing and maintaining the differentiated, nonproliferative state.
CellThe liver-specific transcription factor LF-B1 contains a highly diverged homeobox DNA binding domain
477 Citations1989Monique Frain, Guido W.M. Swart +5 more
The nuclear protein LF-B1 (also referred to as HNF-1) is a transcription activator required for the expression of several liver-specific genes and directs the synthesis in vitro of a protein capable of binding DNA with the same specificity as LF- B1.
CellTwo promoters of different strengths control the transcription of the mouse alpha-amylase gene Amy-1a in the parotid gland and the liver
464 Citations1983Ueli Schibler, Otto Hagenbüchle +2 more
It is shown that two promoters of different strengths are involved in the tissue-specific expression of the alpha-amylase gene Amy-1a in the parotid gland and the liver of mouse.
Genes & DevelopmentThe B-cell-specific Oct-2 protein contains POU box- and homeo box-type domains.
428 Citations1988Roger G. Clerc, Lynn M. Corcoran +3 more
The sequence of a cDNA encoding a B-cell-specific protein, Oct-2, has been determined and it is proposed that the oct-2 gene is expressed as multiple mRNAs that vary in splicing patterns.
CellDBP, a liver-enriched transcriptional activator, is expressed late in ontogeny and its tissue specificity is determined posttranscriptionally
410 Citations1990Christopher R. Mueller, Pascal Maire +1 more
During chemically induced liver regeneration, DBP expression is rapidly down-regulated, suggesting that DBP may be involved in the proliferation control of hepatocytes.
NeuronAntibodies to the period gene product of drosophila reveal diverse tissue distribution and rhythmic changes in the visual system
402 Citations1988Kathleen K. Siwicki, Cheryl Eastman +3 more
The intensity of per-specific staining in the visual system was found to oscillate, defining a free-running circadian rhythm with a peak in the middle of the night.
NatureA human lymphoid- specific transcription factor that activates immunoglobulin genes is a homoeobox protein
385 Citations1988Claus Scheidereit, James A. Cromlish +5 more
The human lymphoid-specific transcription factor OTF-2 contains a homoeodomain that is required for DNA binding and binds specifically to DNA elements that are recognized by Drosophila homoeadomain proteins, suggesting coevolutionary relationships between mammalian and invertebrate homoeODomains proteins and their DNA recognition elements.
CellFactors involved in control of tissue-specific expression of albumin gene
369 Citations1987Silvia Cereghini, Michel Raymondjean +3 more
Proceedings of the National Academy of SciencesTranscriptional control of the murine albumin/alpha-fetoprotein locus during development.
362 Citations1982S M Tilghman, Alexandra Belayew
A model for hepatic expression of the AFP and albumin gene cluster is suggested in which transcription of the two genes is activated simultaneously during differentiation and each gene is thereafter modulated independently in committed cells.
Proceedings of the National Academy of SciencesPurified hepatocyte nuclear factor 1 interacts with a family of hepatocyte-specific promoters.
324 Citations1988Gilles Courtois, Susanne Baumhueter +1 more
The purification of hepatocyte nuclear factor 1 is described and it is demonstrated that it interacts with essential promoter regions of many liver-specific genes, including albumin, alpha-fetoprotein, and transthyretin, which suggests that hepatocytenuclear factor 1 could be one factor necessary for establishing the liver phenotype.
Genes & DevelopmentHNF-1 shares three sequence motifs with the POU domain proteins and is identical to LF-B1 and APF.
266 Citations1990Susanne Baumhueter, Dirk B. Mendel +7 more
It is found that in addition to being present in the liver, HNF is expressed in the kidney, intestine, and spleen, but not in other tissues, which indicates that HNF-1 is a more broadly acting transcription factor than has been indicated by previous work.
CellA glycosylated liver-specific transcription factor stimulates transcription of the albumin gene
246 Citations1989Serge Lichtsteiner, Ueli Schibler
The EMBO JournalPurification of a NF1‐like DNA‐binding protein from rat liver and cloning of the corresponding cDNA.
240 Citations1988Giacomo Paonessa, Fotini Gounari +2 more
Northern analysis reveals several mRNA species present in different combinations in various rat tissues, most likely located within the first 250 amino acids, is highly homologous to the sequence of CTF/NF1 purified from HeLa cells.
Proceedings of the National Academy of SciencesSeveral distinct "CCAAT" box binding proteins coexist in eukaryotic cells.
226 Citations1988Michel Raymondjean, Silvia Cereghini +1 more
It is shown that a "CCAAT" box element present in the rat albumin promoter binds a protein distinct from the CCAAT-binding transcription factor CTF/NFI or from theCBP-binding protein (CBP) previously described.
Journal of Molecular BiologyTissue-specific expression of mouse α-amylase genes
194 Citations1980Ueli Schibler, Mario Tosi +3 more
The results indicate that salivary gland and liver amylase mRNAs are transcribed from identical or very closely related genes which differ from that expressed in the pancreas.
Molecular biology of circadian rhythms
180 Citations2004Amita Sehgal
The author reveals how genetic and Molecular Approaches used to Analyze Rhythms, as well as the physical and molecular characteristics of Plant Circadian Clocks, have changed over time and contributed to the evolution ofcircadianRhythms.
Molecular and Cellular BiologyDeterminants of Rat Albumin Promoter Tissue Specificity Analyzed by an Improved Transient Expression System
161 Citations1987Jean‐Michel Heard, Philippe Herbomel +4 more
Two upstream regions from nucleotides -151 to -119 and from -118 to -94, were required for efficient transcription in H4II cells and each region contained a sequence motif highly conserved among different species that contributed to the overall tissue specificity of rat albumin gene transcription.
Trends in GeneticsClosely watched clocks: molecular analysis of circadian rhythms in Neurospora and Drosophila
105 Citations1990Jay Dunlap
The recent identification and molecular analysis of clock-controlled genes is revealing how biological clocks control gene expression, and may pave the way for the isolation of novel 'clock genes' in the future.
EndocrinologyDaily Variation in Concentration of Plasma Corticosteroid in Hypophysectomized Rats
95 Citations1976Albert Meier
A normal daily variation in plasma corticosteroid concentration does not appear to depend on the periodic release of pituitary hormones.
PubMedTranscriptional activators in hepatocytes.
88 Citations1990Peter F. Johnson
The intent of this article is to summarize some of the emergent information on liver-specific transcriptional activator proteins, especially those that target specific genes by complexing with defined DNA elements located in the vicinity of the transcription unit.
NatureThe Drosophila clock gene per affects intercellular junctional communication
87 Citations1987Thaddeus A. Bargiello, Lino Sáez +4 more
The per locus of Drosophila has been implicated in the control of behavioural rhythms and its effect on junctional communication in the nervous system may explain how per influences behavioural rhythms.
Molecular and Cellular BiologyDeterminants of rat albumin promoter tissue specificity analyzed by an improved transient expression system.
83 Citations1987J M Heard, Philippe Herbomel +4 more
Journal of Molecular BiologyA ubiquitous CCAAT factor is required for efficient in vitro transcription from the mouse albumin promoter
46 Citations1990Jérôme Wuarin, Christopher R. Mueller +1 more
No correlation between the affinity of mutant C-elements for C/EBP and transcriptional activity could be observed in liver nuclear extracts, and a similarly strong correlation of NF-Y-binding with transcription efficiency has also been observed in spleen nuclear extracts.
Journal of Biological ChemistryThyroid hormone and circadian regulation of the binding activity of a liver-specific protein associated with the 5‵-flanking region of the S14 gene
21 Citations1989Norman C.W. Wong, Ana Pérez‐Castillo +3 more
DNase I footprinting analysis revealed that P1 binds to a defined nucleotide sequence, 5'-AAAAGAGCTATTGATTGattGCCTGCA-3', located between -310 and -288 in the S14 gene.