Induction of interleukin-1β mRNA in adult rat brain
Molecular Brain ResearchPublished 1 January 1991
Gerald A. Higgins, John A. Olschowka
Citations135
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
These studies show that IL-1β mRNA can be induced to high levels within the CNS as a consequence of exposure to potent stimulators of macrophaga activation.
Abstract
The ability of the central nervous system to produce the cytokine interleukin-1 beta (IL-1 beta) in response to challenge by activators of the mononuclear phagocyte system has been examined in vivo. Unilateral injection of a mixture of gamma-interferon (IFN-gamma) and lipopolysaccharide (LPS) into the forebrain of adult rats induced expression of IL-1 beta mRNA. In situ hybridization of IL-1 beta mRNA showed a gradient of cellular hybridization, which was most intense at the site of IFN-gamma/LPS injection. The reverse transcription--polymerase chain reaction (RT-PCR) was used to demonstrate the presence of IL-1 beta mRNA in normal rat brain, and to confirm increases in IL-1 beta mRNA levels following IFN-gamma/LPS injection. These studies show that IL-1 beta can be induced to high levels within the CNS as a consequence of exposure to potent stimulators of macrophage activation.
Keywords
Immunology and MicrobiologyNeuroscience
Proceedings of the National Academy of SciencesBrain interleukin 1 and S-100 immunoreactivity are elevated in Down syndrome and Alzheimer disease.
1,773 Citations1989W. Sue T. Griffin, Laura Stanley +6 more
The data suggest that increased expression of S-100 in Down syndrome, resulting from duplication of the gene on chromosome 21 that encodes the beta subunit ofS-100, may be augmented by elevation of interleukin 1, which may promote the astrogliosis in Alzheimer disease.
Annual Review of ImmunologyThe Cell Biology of Macrophage Activation
1,611 Citations1984Dolph O. Adams, Thomas A. Hamilton
NatureCloning, sequence and expression of two distinct human interleukin-1 complementary DNAs
1,586 Citations1985Carl J. March, Bruce Mosley +11 more
Two distinct but distantly related complementary DNAs encoding proteins sharing human interleukin-1 (IL-1) activity (termed IL-lα and IL-1β), were isolated from a macrophage cDNA library.
The FASEB JournalBiology of interleukin 1
1,539 Citations1988Charles A. Dinarello
IL 1 is a highly inflammatory molecule and stimulates the production of arachidonic acid metabolites and acts synergistically with other cytokines, particularly tumor necrosis factor.
Proceedings of the National Academy of SciencesNucleotide sequence of human monocyte interleukin 1 precursor cDNA.
1,044 Citations1984Philip E. Auron, Andrew C. Webb +5 more
The cDNA nucleotide sequence suggests that IL-1 is initially translated as a precursor molecule that is subsequently processed into the 15,000-20,000 Mr protein usually associated with IL- 1 activity.
The Journal of Experimental MedicineInterleukin 1 of the central nervous system is produced by ameboid microglia.
864 Citations1986Dana Giulian, T J Baker +2 more
It is proposed that the proliferation of astroglia in specific brain regions may be regulated by the signaled release of IL-1 from activated microglial cells.
NatureCloning and expression of murine interleukin-1 cDNA in Escherichia coli
823 Citations1984Peter T. Lomedico, Ueli Gubler +8 more
The cloning, sequence analysis and expression of murine IL-1 cDNA in Escherichia coli reveals a polypeptide precursor of 270 amino acids that may play a major role in the initiation and amplification of immune and inflammatory responses through its action on these diverse cell populations.
Proceedings of the National Academy of SciencesProduction of tumor necrosis factor and other cytokines by astrocytes stimulated with lipopolysaccharide or a neurotropic virus.
787 Citations1989Andrew P. Lieberman, Paula M. Pitha +2 more
It is proposed that astrocytes and the cytokines they produce may play a significant role in the pathogenesis of immunologically and/or virally mediated CNS disease, in CNS intercellular communication, and in the interactions between the nervous and immune systems.
ScienceInterleukin-1 Immunoreactive Innervation of the Human Hypothalamus
764 Citations1988Christopher D. Breder, Charles A. Dinarello +1 more
Results indicate that IL-1 may be an intrinsic neuromodulator in central nervous system pathways that mediate various metabolic functions of the acute phase reaction, including the body temperature changes that produce the febrile response.
Trends in NeurosciencesMacrophages and microglia in the nervous system
742 Citations1988V. Hugh Perry, Siamon Gordon
The possible functions of resident and recruited macrophages in the developing and adult nervous system are reviewed and what contribution these cells might make to repair mechanisms in the central and peripheral nervous systems are examined.
Proceedings of the National Academy of SciencesInterleukin 1 regulates synthesis of amyloid beta-protein precursor mRNA in human endothelial cells.
591 Citations1989Dmitry Goldgaber, H. Harris +6 more
It is suggested that IL-1 upregulates APP gene expression in HUVEC through a pathway mediated by protein kinase C, utilizing the upstream AP-1 binding site of the APP promoter.
Journal of NeuroscienceInterleukin-1 injected into mammalian brain stimulates astrogliosis and neovascularization
589 Citations1988Dana Giulian, Julia Woodward +3 more
Recombinant forms of IL-1 injected into the cerebral cortex of adult rats elicit not only astrogliosis but also new blood vessel growth, which suggest thatIL-1-secreting inflammatory cells may mediate wound healing in the CNS.
Journal of NeuroscienceThe role of mononuclear phagocytes in wound healing after traumatic injury to adult mammalian brain
502 Citations1989Dana Giulian, J Chen +3 more
The observations show that mononuclear phagocytes play a major role in wound healing after CNS trauma with some events controlled by secretion of cytokines, and certain classes of immunosuppressive drugs may be useful in the treatment of acute brain injury.
Journal of NeuroscienceInterleukin-1 is an astroglial growth factor in the developing brain
448 Citations1988Dana Giulian, DG Young +3 more
In vitro studies indicate that ameboid microglia, a class of brain mononuclear phagocytes, are the likely source of IL-1, which is found to be an astroglial growth factor during development of the mammalian brain.
European Journal of ImmunologyMonokine production by microglial cell clones
377 Citations1989Marco Righi, Lucia Mori +6 more
The immortalized with oncogenic retroviruses primary brain cell cultures from mouse embryos and clones of microglial cells that have been characterized suggest that early resident microglia cells might play an important role in developmental processes and in the adult brain.
Brain ResearchImmunoreactive interleukin-1β localization in the rat forebrain
344 Citations1990Ronald M. Lechan, Roberto Toni +5 more
These studies demonstrate that IL-1 beta is part of a diffuse intrinsic neural system in the rat central nervous system, associated with regions involved with hypophysiotropic, autonomic, limbic and extrapyramidal functions.
The Journal of BiochemistryMolecular Cloning and Expression of Rat Interleukin-1α cDNA
327 Citations1989Tsutomu Nishida, Naoki Nishino +7 more
Recombinant rat IL-1 alpha produced in COS cells or E. coli has cytotoxic activity against the human melanoma cell line A375S1 (GIF activity), which suggests that GIF activity is common toIL-1s derived from various sources.
Journal of Neuroscience ResearchBrain macrophages synthesize interleukin‐1 and interleukin‐1 mRNAs in vitro
288 Citations1988Emmanuelle Hetier, J. Ayala +5 more
ScienceRelation of Neuronal APP-751/APP-695 mRNA Ratio and Neuritic Plaque Density in Alzheimer's Disease
253 Citations1990Steve A. Johnson, Thomas H. McNeill +2 more
The increase in APP-751/APP-695 mRNA provides a molecular marker for regional variations in plaque density between individuals diagnosed with Alzheimer's disease by the commonly used composite criteria.
Oxford University Press eBooksIn situ hybridization : applications to neurobiology
250 Citations1987Karen L. Valentino, James Eberwine +1 more
In situ hybridization for mapping the neuroanatomical distribution of novel brain mRNAs and methods for hybridization and quantitation of mRNA in individual brain cells are proposed.
Proceedings of the National Academy of SciencesDifferential regulation of amyloid-beta-protein mRNA expression within hippocampal neuronal subpopulations in Alzheimer disease.
176 Citations1988Gerald A. Higgins, David A. Lewis +6 more
The neuroanatomical distribution of amyloid-beta-protein mRNA within neuronal subpopulations of the hippocampal formation in the cynomolgus monkey, normal aged human, and patients with Alzheimer disease is mapped.
Experimental NeurologyNGF receptor gene expression is decreased in the nucleus basalis in Alzheimer's disease
159 Citations1989Gerald A. Higgins, Elliott J. Mufson
In situ hybridization is used to show that NGF-receptor (NGF-R) mRNA-positive neurons are lost within all divisions of the nucleus basalis of Meynert in AD patients as compared to normal aged controls.
The Journal of ImmunologyDetection of IL-1 <i>alpha</i> and IL-1 <i>beta</i> gene expression by in situ hybridization. Tissue localization of IL-1 mRNA in the normal C57BL/6 mouse.
121 Citations1988László Takács, Éva Kovács +3 more
The production of IL-1 mRNA in the thymus and in the bone marrow suggests thatIL-1 is available to play physiologic roles in T cell differentiation and in hemopoiesis.
The Journal of ImmunologyCharacterization of lipopolysaccharide-induced macrophage gene expression.
91 Citations1988Charles S. Tannenbaum, T J Koerner +2 more
Six clones hybridizing with mRNA sequences present in LPS-treated cells but not in controls were selected and appear to encode inducible macrophage genes that are distinct from one another as well as from a selection of previously described early genes.
NeuronThe Alzheimer amyloid precursor-related transcript lacking the β/A4 sequence is specifically increased in Alzheimer's disease brain
77 Citations1990Rachael L. Neve, Joseph Rogers +1 more
It is demonstrated that a specific spliced form of mRNA that is transcribed from the APP gene and that lacks the beta/A4 sequence is elevated in the nucleus basalis, occipitotemporal cortex, and parahippocampal gyrus in Alzheimer's disease brain relative to controls.
Journal of Leukocyte BiologyHuman Monocyte Production of Interleukin-1: Parameters of the Induction of Interleukin-1 Secretion by Lipopolysaccharides
77 Citations1986Robert Newton
The results demonstrate that when blood monocytes are prepared under low endotoxin conditions, they do not spontaneously secrete IL‐1 activity, which appears to be sensitive to fg/ml levels of Salmonella minnesota LPS, while appreciable secretion of this activity by monocytes requires pg/ ml levels.
The Journal of ImmunologyDemonstration by in situ hybridization of dissimilar IL-1 <i>beta</i> gene expression in human alveolar macrophages and blood monocytes in response to lipopolysaccharide.
69 Citations1988Jean‐François Bernaudin, Kiyomi Yamauchi +4 more
There is a heterogeneity among population of mononuclear phagocytes in their ability to express the gene forIL-1 beta, which could explain the differences observed in IL-1 production.
The Journal of ImmunologyIFN-gamma and IFN-<i>beta</i> independently stimulate the expression of lipopolysaccharide-inducible genes in murine peritoneal macrophages.
62 Citations1989Thomas A. Hamilton, N Bredon +2 more
The results suggest that IFN and LPS act to modulate early gene expression by the generation of at least three overlapping but distinct signaling pathways.
Nucleic Acids ResearchThe murine interleukin 1β gene: structure and evolution
62 Citations1986John L. Telford, G Macchia +4 more
The comparative analysis between the murine IL-1 beta and the humanIL-1 alpha genes shows extreme conservation of the aminoacids at the exon junctions, which suggests that these genes have diverged from a common ancestor.
Neurobiology of AgingFocusing on IL1-promotion of β-amyloid precursor protein synthesis as an early event in Alzheimer's disease
36 Citations1989Arthur J. Blume, Michael P. Vitek
A rationale for increased synthesis of beta-amyloid peptide precursor (APP) protein in Alzheimer's disease (AD) is developed in which Interleukin-1 (IL-1) plays a key role.
Immunologic ResearchInterleukin-1 and the response to injury
29 Citations1989Elliot Kaplan, C A Dinarello +1 more
Manipulation of IL-1 mediated effects might be of therapeutic utility in the management of trauma in the future and may lead to normal immunologic response and for wound healing.
PubMedAnalysis of T cell receptor transcripts using the polymerase chain reaction.
14 Citations1990Matthew E. Roth, Michael J. Lacy +2 more
Several strategies that use the polymerase chain reaction (PCR) to analyze very rapidly the structure of TCRs are described, as are the advantages of using the PCR to study TCR diversity.