The structure of nuclear polyhedrosis viruses
VirologyPublished 1 October 1972
K.A. Harrap
Citations120
SJR quartileQ2
SJR score0.74
SNIP0.60
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
A model is proposed for the subunits of the inclusion body protein lattice which explains the arrangement seen both in section and in aggregates of protein from alkali-treated polyhedra.
Abstract
The inclusion bodies of two nuclear polyhedrosis viruses have been studied electron microscopically. The presence of a structurally distinct layer has been confirmed at the surface of the polyhedral inclusion body. The structure is visible in section as an electron-dense layer and can also be observed when separated from suspensions of alkali-treated polyhedra using negative staining techniques. A model is proposed for the subunits of the inclusion body protein lattice which explains the arrangement seen both in section and in aggregates of protein from alkali-treated polyhedra.
Keywords
MedicineAgricultural and Biological SciencesEnvironmental Science
The Journal of Cell BiologyTHE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY
25,027 Citations1963Edward S. Reynolds
The stain reported here differs from previous alkaline lead stains in that the chelating agent, citrate, is in sufficient excess to sequester all lead present, and is less likely to contaminate sections.
PROTOPLASMAThe greening process in plastids
192 Citations1965B. E. S. Gunning
Plastids in etiolatedAvena leaves were studied by electron microscopy of thin sectioned material fixed in glutaraldehyde and osmium tetroxide and embedded in Epon to conclude that this type of organisation is widespread in plants.
The Journal of Cell BiologyELECTRON MICROSCOPE STUDIES ON DEVELOPING CRAYFISH OOCYTES WITH SPECIAL REFERENCE TO THE ORIGIN OF YOLK
191 Citations1963H. W. Beams, Richard G. Kessel
The endoplasmic reticulum is composed, in places, of stacks of parallel cisternae which are limited by membranes having great numbers of ribosomes attached to their outer surface, a morphological condition suitable to serve as the basis for an active transport system.
Zeitschrift für Naturforschung BDie Isolierung des Polyeder-Virus und die Natur der Polyeder
170 Citations1947G. H. Bergold
Journal of Ultrastructure ResearchElectron microscopic observations on granulosis virus entry, uncoating and replication processes during infection of the midgut cells of Trichoplusia ni
155 Citations1971M D Summers
The results of this study suggest that the viral envelope is lost at, or during, the entry process since only nonenveloped nucleocapsids were seen in cells shortly after infection, and it is suggested that virus progeny are released into the insect hemocoel by such processes.
The Journal of Experimental MedicineCULTIVATION OF THE VIRUS OF GRASSERIE IN SILKWORM TISSUE CULTURES
127 Citations1935William Trager
In tissue cultures, strains of silkworm grasserie virus were maintained in successive passages up to the number of ten and typical polyhedral bodies formed in the cells of infected cultures.
Journal of General VirologyA Possible Infection Pathway in the Development of a Nuclear Polyhedrosis Virus
78 Citations1968K.A. Harrap, J. S. Robertson
Larvae of the small tortoiseshell butterfly, Aglais urticae (Lepidoptera: Nymphalidae), were infected orally with a nuclear polyhedrosis virus and no incorporation of the virus particles into the polyhedral inclusion body was observed although both virus particles and crystalline polyhedron protein were present.
NatureThe Virogenic Stroma in Nuclear and Cytoplasmic Polyhedroses
78 Citations1956N. Xeros
It is found that in the larval fat body and midgut cells of Lepidoptera and in the midguts cells of Hymenoptera infected with nuclear polyhedroses proteinaceous virogenic stromata form de novo in the nuclear sap.
VirologyCell infection by a nuclear polyhedrosis virus
76 Citations1970K.A. Harrap
Infection through the microvilli of the gut columnar cells is suggested as the initial stage of infection by a nuclear polyhedrosis virus, and the importance of the virus envelope in the infection of the cell is discussed.
Journal of VirologyApparent In Vivo Pathway of Granulosis Virus Invasion and Infection
63 Citations1969Max D. Summers
The invasion and ensuing replication of an insect granulosis virus in Trichoplusia ni is described.
Canadian Journal of Research/Canadian journal of researchTHE MULTIPLICATION OF INSECT VIRUSES AS ORGANISMS
58 Citations1950G. H. Bergold
Electron micrographs of purified preparations of four different insect viruses indicate the presence of morphologically different forms, which are probably stages of multiplication.
VirologyThe structure of nuclear polyhedrosis viruses
57 Citations1972K.A. Harrap
The synthesis and envelopment of the virus nucleocapsid and stages in the formation of the polyhedral inclusion bodies are described and comparisons made with the results of an earlier study of the structure of these viruses when negative staining techniques were used.
Journal of Ultrastructure ResearchThe molecular structure of some insect virus inclusion bodies
45 Citations1963G. H. Bergold
Electron microscopic investigation have revealed very regular dot and line patterns in different crystalline insect virus inclusion bodies, the so-called polyhedra and capsules, which indicated that there are six relative points of special attraction on the surface of each molecule, which leads to the cubic system.
Journal of Invertebrate PathologyThe purification and properties of inclusion body protein of the granulosis virus of Pieris brassicae
45 Citations1972J.F. Longworth, J. S. Robertson +1 more
Inclusion bodies of the granulosis virus of Pieris brassicae were dissolved in alkali, and three components were obtained-inclusion body protein, enveloped virus particles, and the inclusion body envelope, which showed that two proteins were present in solutions of inclusion body protein.
HilgardiaThe development of an insect virus within cells of its host
30 Citations1953Kenneth M. Hughes
Stages in the development of an insect virus are shown in this study and information on theDevelopment of polyhedral bodies is presented.
Journal of Invertebrate PathologyIsolation and some properties of components of nuclear polyhedra from the cabbage looper, Trichoplusia ni
27 Citations1971H. A. Scott, S. Y. Young +1 more
Nuclear polyhedra obtained from diseased cabbage looper were digested with sodium carbonate-saline buffer, pH 11.0, and formed 3 general zones when subjected to density gradient centrifugation.
Journal of VirologySeparation and Structure of Components of Nuclear Polyhedrosis Virus of the Silkworm
27 Citations1971Toshiyuki Khosaka, Michio Himeno +1 more
Morphology of structural components of nuclear polyhedrosis virus (NPV) particles of the silkworm was studied by electron microscope using negative staining, indicating the particles to be the degradation product of the virus particles.
Journal of Invertebrate PathologyDensity-gradient centrifugation of the virions liberated from Rachiplusia ou nuclear polyhedra
25 Citations1970C.Y. Kawanishi, J.D. Paschke
Physical evidence suggests that the R. ou nuclear polyhedrosis virus produces multiploid virions, and that the virions are sedimenting according to the number of nucleocapsids within.
Journal of Cell ScienceMoiré patterns in electron micrographs of a bacterial membrane
23 Citations1966Audrey M. Glauert
A regular hexagonal array of morphological units is observed in negatively stained preparations of a membrane from the surface of the spherical bacterium Micrococcus radiodwans.
Journal of Invertebrate PathologyElectron-microscope investigation of the structure of the nuclear-polyhedrosis virus of the silkworm, Bombyx mori
23 Citations1967Э. А. Козлов, I.P. Alexeenko
Electron micrographs of intact particles of the nuclear-polyhedrosis virus of the silkworm, Bombyx mori, and of those particles treated with chloroform or urea have revealed new details of their inner structure, and a hypothetical model of the structure of the virus is proposed.
European Journal of Plant PathologyElectron microscopy of DNA-cores in nuclear polyhedral viruses
14 Citations1965M.B. Ponsen
Electron micrographs show the presence of DNA-cores in the virus rods isolated from nuclear polyhedral bodies from Barathra brassicae, Adoxophyes reticulana andOrgyia antiqua.
European Journal of Plant PathologyElectron microscope observations on the structure of membranes from nuclear polyhedral viruses
13 Citations1965M.B. Ponsen, S. Henstra +1 more
Electron micrographs show the presence of the polyhedral, the outer, and the inner membranes occurring in nuclear polyhedral bodies from Malacosoma neustria, Barathra brassicae and Adoxophyes reticulana.
European Journal of Plant PathologyElectron microscope observations of nuclear polyhedra fromMalacosoma neustria (Lepidoptera: Lasiocampidae
10 Citations1964M.B. Ponsen, S. Henstra +1 more
The nuclear polyhedral bodies fromMalacosoma neustria are enclosed within a membrane and the virus rods are closely packed by the intimate membrane and between the intimate and the developmental membrane is a space.