THE PREPARATION OF 131I-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY
Biochemical JournalPublished 1 October 1963Open access
FC Greenwood, WM Hunter, J. Glover
Citations10,192
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
The loss of immunological reactivity at high specific radioactivities or at high levels of chemical substitution with STAI/sup 127/!iodine is demonstrated.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
Journal of Biological ChemistryPROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
318,326 Citations1951OliverH. Lowry, NiraJ. Rosebrough +2 more
Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
NaturePreparation of Iodine-131 Labelled Human Growth Hormone of High Specific Activity
6,958 Citations1962W. M. Hunter, F. C. Greenwood
Current procedures for the immunological assay of protein hormones in human plasma require the routine preparation of hormones labelled with iodine-131 of high specific activity, and this work demonstrates the importance of knowing the carrier and removal status of iodine.
Journal of Clinical InvestigationIMMUNOASSAY OF ENDOGENOUS PLASMA INSULIN IN MAN
3,035 Citations1960Rosalyn S. Yalow, Solomon A. Berson
NatureEfficient Trace-labelling of Proteins with Iodine
2,559 Citations1958A. S. McFarlane
Values greater than 50 per cent can be obtained by adding oxidizing agents to liberate iodine from iodide, but most if not all of these appear to affect adversely the properties of the labelled protein.
Obesity ResearchIMMUNOASSAY OF ENDOGENOUS PLASMA INSULIN IN MAN
460 Citations1996Rosalyn S. Yalow, Solomon A. Berson
The insulin concentration in plasma has been estimated from the degree of hypoglycemia produced in hypophysectomized, adrenalectomization, alloxan-diabetic rats, and from the increased oxidation of glucose-1-C14 by the rat epididymal fat pad.
Journal of Clinical InvestigationSTUDIES ON HUMAN GROWTH HORMONE. I. A RADIO-IMMUNOASSAY FOR HUMAN GROWTH HORMONE*
183 Citations1962Robert D. Utiger, Mary L. Parker +1 more
Experimental Biology and MedicineGlucagon Antibodies and Their Use for Immunoassay for Glucagon.
178 Citations1959R. H. Unger, Anna M. Eisentraut +4 more
Non-precipitating antibodies to glucagon have been produced in rabbits and detected by both Berson-Yalow and Skom-Talmadge technics, and a basis for an immunoassay for glucagon appears to have been established.
ScienceAntigens in Insulin Determinants of Specificity of Porcine Insulin in Man
72 Citations1963Solomon A. Berson, Rosalyn S. Yalow
Porcine insulin, which is distinguished from human insulin only in the amino acid at the C terminal of the B chain, is antigenic in man; thus, the antigenic determinant of porcine insulin is located in a part of the molecule where the amino acids sequence is the same as it is in the corresponding parts of the human insulin molecule.
NatureTrace-labelling of Insulin with Iodine
36 Citations1961Ellis Samols, H.S. Williams
A new technique for the immunological assay of human plasma insulin requires a radioactive bovine insulin of very high specific activity to be used.
Experimental Biology and MedicineProduction of Glucagon Antibodies and their Role in Metabolism and Immunoassay of Glucagon.
29 Citations1961G M Grodsky, T. Hayáshida +2 more
The antibody content of sera from rabbits treated intermittently with free glucagon or glucagon conjugated with ovalbumin was measured at intervals during a 2-year period to find out whether glucagon-I131 could be dissociated from its antibody at acid pH.