Nucleotide sequence of the Bacillus subtilis ribosomal RNA operon, rrnB
GenePublished 1 January 1985
Christopher J. Green, George C. Stewart, Mary Ann Hollis, Barbara S. Vold, Kenneth F. Bott
Citations212
SJR quartileQ2
SJR score0.68
SNIP0.72
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TL;DR
The primary sequence of DNA covering a complete ribosomal RNA (rRNA) operon from Bacillus subtilis, designated rrnB has been elucidated and represents the first report of the entire sequence of an rRNA operon to be reported from B. subtILis or from any Gram-positive organism.
Abstract
The primary sequence of DNA covering a complete ribosomal RNA (rRNA) operon from Bacillus subtilis, designated rrnB has been elucidated. Following a set of tandem promoters, rrnB encodes: (i) a 16S and a 23S rRNA determinant with no tRNA spacer region in between; (ii) a 5S rRNA determinant; and (iii) 21 contiguous tRNA species; before (iv) two characteristic terminator hairpins are found. More than 7 kb are included within this operon, which maps between aroG and thr5 on the B. subtilis chromosome. This represents the first report of the entire sequence of an rRNA operon from B. subtilis or from any Gram-positive organism.
Keywords
Biochemistry, Genetics and Molecular BiologyEnvironmental Science
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