The Histones, their Interactions with DNA, and Some Aspects of Gene Control
Novartis Foundation symposiumPublished 1 January 1969
E.W. Johns
Citations25
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Abstract
The prelims comprise: Fractionation and Characterization of Histones Interactions between Histones and DNA Histones and Gene Control The Avian Erythrocyte Specific Histone Summary
Keywords
Biochemistry, Genetics and Molecular Biology
Biochemical JournalStudies on histones. 7. Preparative methods for histone fractions from calf thymus
1,039 Citations1964EW Johns
Proceedings of the National Academy of SciencesTHE STRUCTURE OF THE DNA-ACRIDINE COMPLEX
720 Citations1963Leonard S. Lerman
Proceedings of the National Academy of SciencesHISTONE, A SUPPRESSOR OF CHROMOSOMAL RNA SYNTHESIS
587 Citations1962Ru-chih C. Huang, James Bonner
It will be shown that the DNA of pea embryo chromatin is present in at least two forms, namely, as DNA itself and as DNA bound in nucleohistone complex, and that DNA fully complexed with histone is inactive in the support of DNA-dependent RNA synthesis.
Journal of Molecular BiologyOrgan-specific restriction of transcription in mammalian chromatin
556 Citations1968John Paul, Raymond Gilmour
It is concluded that histones can mask DNA in chromatin and prevent it from acting as a template, but this effect is nonspecific.
Journal of Molecular BiologyThe isolation and properties of deoxyribonucleoprotein particles containing single nucleic acid molecules
483 Citations1959Geoffrey Zubay, Paul Doty
It is shown that a large fraction of the histone can be accommodated in sections having the α-helical configuration in the large groove of the double-stranded deoxyribonucleic acid helix and that such a model is consistent with the observations made thus far.
Proceedings of the National Academy of SciencesON THE ROLE OF HISTONES IN REGULATING RIBONUCLEIC ACID SYNTHESIS IN THE CELL NUCLEUS
411 Citations1963V. G. Allfrey, Virginia C. Littau +1 more
NatureCell Specificity of Histones
365 Citations1950Edgar Stedman, Ellen Stedman
It is suggested that each nucleus possesses a basic protein characteristic of the type of cell of which it forms part, and the histones isolated from cell nuclei of other species are examined.
Proceedings of the National Academy of SciencesISOLATION OF THE λ PHAGE REPRESSOR
286 Citations1967Mark Ptashne
Biochemical JournalThe electrophoresis of histones in polyacrylamide gel and their quantitative determination
270 Citations1967E.W. Johns
A method has been developed for the quantitative determination of the separated histone fractions by measuring the colour yields of dye-histone complexes formed in the gel, and the relative mobilities with respect to a marker protein, bovine plasma albumin.
Journal of Molecular BiologyPhysical and biological properties of soluble nucleohistones
245 Citations1964Ru Chih C. Huang, James Bonner +1 more
Soluble nucleohistones have been reconstituted by gradient dialysis from individual histone fractions and a common preparation of DNA, and the preparations appear to consist to a substantial degree of individual DNA molecules fully complexed with histone.
Journal of Cell ScienceThe reactivation of the red cell nucleus
233 Citations1967H. Harris
It is considered that changes in nuclear volume may regulate not only the amount of RNA made in the nucleus but also the areas of chromatin on which it is made.
Journal of Biological ChemistryThe Resolution of Four Lysine-rich Histones Derived from Calf Thymus
231 Citations1966Joseph M. Kinkade, R. David Cole
The most effective resolution of lysinerich histones, however, was obtained by chromatography on Amberlite IRC-50 with a gradient of guanidinium chloride in 0.1 m phosphate (pH 6.8); this technique was especially powerful in detecting degradation products in the preparations.
Journal of Biological ChemistrySpecies and Organ Specificity in Very Lysine-rich Histones
227 Citations1968Michael Bustin, R. David Cole
The chromatographic resolution of the subfractions of the very lysine-rich histones, their amino acid compositions, and their relative mobilities on polyacrylamide gels suggest that the whole very lyridine-rich complement of any species consists of a moderately large number of molecular types.
Biochemical JournalFurther fractionations of histones from calf thymus
198 Citations1962E.W. Johns, J. A. V. Butler
NatureSuper-helical Model for Nucleohistone
190 Citations1967J. F. Pardon, M. H. F. Wilkins +1 more
A coiled-coil structure for nucleohistone is discussed and new experimental evidence that is compatible with it is found that iscompatible with it.
Biochemical JournalA method for the selective extraction of histone fractions f2(a)1 and f2(a)2 from calf thymus deoxyribonucleoprotein at pH7
172 Citations1967E.W. Johns
Modifications of existing electrophoretic methods are described that enable these fractions to be more easily characterized.
Journal of Molecular BiologyProperties of chromosomal nucleohistone
168 Citations1963James Bonner, Ru-chih C. Huang
The deoxyribonucleohistone component may be physically separated from the other components of pea embryo chromatin because of its higher melting temperature, in its histone/DNA mass ratio and in its inactivity in the support of DNA-dependent RNA synthesis.
BiochemistryOn the Similarity of Plant and Animal Histones<sup>*</sup>
167 Citations1966Douglas M. Fambrough, James Bonner
BiochemistryQuantitative distribution of histone components in the pea plant
166 Citations1968Douglas M. Fambrough, Frank K. Fujimura +1 more
Biochemical JournalImproved fractionations of arginine-rich histones from calf thymus
166 Citations1960E.W. Johns, D. M. P. Phillips +2 more
Progress in nucleic acid research and molecular biologyProteins of the Cell Nucleus
162 Citations1967Lubomir S. Hnilica
It is found that the amino acid composition of nucleolar proteins from the mammalian tissues is similar to the composition of the corresponding fractions isolated from the whole nuclei.
Proceedings of the National Academy of SciencesHistone-bound RNA, a component of native nucleohistone.
158 Citations1965Ru Chih C. Huang, James Bonner
It is found that native histone molecules as they occur in the nucleohistone component of pea bud chromatin contain RNA molecules chemically linked to them, which constitutes a new class of RNA, differing from other classes in base composition and in chain length.
Biochemical JournalA FRACTIONATION OF THE HISTONES OF GROUP F2A FROM CALF THYMUS
157 Citations1965DMP PHILLIPS, E.W. Johns
The calf-thymus histone group F2a has been separated into two subfractions by stepwise precipitation with acetone from acid solution and both fractions have a very low content of N-terminal amino acids and contain acetyl groups.
Experimental Cell ResearchInitial changes of deoxyribonucleoprotein and synthesis of nucleic acid in phytohemagglutinine-stimulated human leucocytes
148 Citations1965D. Killander, R RIGLERJR
Journal of Molecular BiologyX-ray diffraction studies of the molecular structure of nucleohistone and chromosomes
147 Citations1959M. H. F. Wilkins, Geoffrey Zubay +1 more
A lipid, resembling sphingomyelin, is associated with the histone in nucleohistone and gives rise to strong diffraction superimposed on the pattern due to nucleohistsone itself, which is recognisable at 98 % relative humidity.
Biochemical JournalThe <i>N</i>-terminal groups of calf-thymus histones
109 Citations1958D. M. P. Phillips
International Journal of Radiation Biology and Related Studies in Physics Chemistry and MedicineThe Cell Nucleus–Metabolism and Radiosensitivity
94 Citations1966Alma Howard
Biochemical Journal‘GLUCOSE 6-PHOSPHATE-DEHYDROGENASE’ ACTIVITY AND THIOL CONTENT OF THYMUS NUCLEI FROM CONTROL AND X-IRRADIATED RATS
88 Citations1963H DEAKIN, MG ORD +1 more
A study was made of the thiol groups present in nuclei froin rat thymus gland, finding that 70% was associated with arginine-rich histones andLinoleic acid peroxide, in concentrations approximately equimolar to theThiol concentration, decreased the thiola content of the nuclei.
Biochemical JournalThe electrophoresis of histones and histone fractions on starch gel
86 Citations1961E.W. Johns, DMP PHILLIPS +2 more
It is reasonable to conclude from these results that phosphorylcholine and phosphorylethanol-amine are quantitatively important constituents of the phosphoric acid derivatives extractable from goat placenta by perchloric acid.
Journal of Molecular BiologyPreparation and properties of RNA-polymerase particles
86 Citations1964E. Fuchs, Wolfram Zillig +2 more
Journal of Biological ChemistryCalf and Pea Histone IV
84 Citations1968R J DeLange, D M Fambrough +2 more
The identity of this region and the similar amino acid compositions suggest that the remainder of the sequence of this histone from both the animal and the plant must be closely related.
Biochemical and Biophysical Research CommunicationsThe relative abllity of reconstituted nucleohistones to allow DNA-dependent RNA synthesis
82 Citations1963J. Roger Hindley
This communication is concerned with the relative ability of reconstituted DNA histone complexes to permit DNA dependent RNA synthesis.
NatureConformational Investigations of Histones
79 Citations1965E. Morton Bradbury, Colyn Crane‐Robinson +3 more
The apparently conflicting results of the experiments on the repression of DNA-dependent RNA synthesis are not in agreement with one another, and the inhibitory capacities of the various histone fractions appear to depend on the method of preparation of the recombined nucleohistone, particularly the relative proportions of histone and DNA used for recombination.
Analytical BiochemistryThe heterogeneity of arginine-rich histones
71 Citations1965Lubomir S. Hnilica, Lloyd G. Bess
Results of the amino acid analysis, of the NH 2 terminal amino acid determination, and of the analysis of tryptic peptides (fingerprinting), indicated that these fractions are polymers or aggregates of a basic molecular unit.
Biochimica et Biophysica Acta (BBA) - Protein StructureA quantitative comparison of histones from immature and mature erythroid cells of the duck
56 Citations1969Claire Dick, E.W. Johns
The histone fraction unique to avian red blood cells was characterized electrophoretically on the gel and the five other histone fractions found in most tissues were also present in the blood histones.
Journal of Biological ChemistryComparison of Histones Obtained from Mammary Gland at Different Stages of Development and Lactation
47 Citations1968Robert H. Stellwagen, R. David Cole
The very lysine-rich histone from mammary gland approached that of thymus in amino acid composition when minor histone fractions were removed with 5% trichloracetic acid.
Journal of Molecular BiologyObservations on the structure of RNA polymerase and its attachment to DNA
45 Citations1969Martin Lubin
In both preparations polymerase appeared to be reasonably homogeneous in size, porous and without obvious planes of symmetry or regularly repeating subunits, and in both preparations hexagons, striped rectangles and other symmetrical structures are apparently contaminants of impure preparations.
Biochemical JournalInteractions of calf-thymus histone fractions in aqueous solution with 8-anilinonaphthalene-1-sulphonic acid
42 Citations1966DJR Laurence
The interactions of histone fractions with 8-anilinonaphthalene-1-sulphonic acid were investigated by fluorimetry and spectrofluorimetric and the results were interpreted with the aid of equilibrium-dialysis techniques to discuss the close relationship between binding and aggregation and the possible role of non-polar interactions as determined by the balance between polar and non-Polar amino acids in the hist one fractions.
Proceedings of the National Academy of SciencesNucleohistone as a primer for RNA synthesis.
41 Citations1965Barbara Sonnenberg, Geoffrey Zubay
Proceedings of the National Academy of SciencesSTRUCTURE OF THE CHROMATIN IN SEA URCHIN SPERM
40 Citations1965Alberto J. Solari
It is shown that if z, co real, is a characteristic root of A of multiplicity w, then lim (z - iwo)lc'(zI - A)b # 0.
Biochemical JournalIdentification of some peptides from an arginine-rich histone and their bearing on the structure of deoxyribonucleohistone
37 Citations1962DMP PHILLIPS, P Simson
Biochemical JournalInteractions between the lysine-rich histone F1 and deoxyribonucleic acid
29 Citations1969E.W. Johns, S. Forrester
Bivalent cations are shown to be more effective in dissociating the DNA-histone complex and can be selectively removed from deoxyribonucleoprotein with 0.1m-magnesium chloride.
Journal of Molecular BiologyReconstitution of nucleohistone from homologous and heterologous components
23 Citations1968R.A. Garrett
Biochimica et Biophysica Acta (BBA) - Protein StructureThe C-terminal amino acids and sequences of calf and rat thymus histones
16 Citations1969D. M. P. Phillips, P Simson
The five ratThymus histones were found to have the same C-terminal amino acids as the corresponding calf thymus fractions, and the lowest weights found were near the molecular weights given by other methods.
Biochemical JournalStudies on histones. 8. A degradation product of lysine-rich histone
9 Citations1964EW Johns