Differential activation of transcription of chromatin by non-histone fractions
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein SynthesisPublished 1 March 1972
Nina C. Kostraba, T.Y. Wang
Citations83
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TL;DR
It is concluded that such non-histone protein fractions are capable of differentially augmenting transcription in vitro from DNA sequences of chromatin.
Abstract
In the RNA polymerase reaction templated by rat liver or Walker tumor chromatin, addition of a non-histone protein fraction that is homologous to the tissue of chromatin stimulates RNA synthesis in vitro to a greater extent than the heterologous non-histone protein fraction. DNA · RNA hybridization experiments of the transcribed RNA show (1) that RNA synthesized from activated chromatin is transcribed from additional DNA sequences; and (2) that the homologous non-histone protein fraction activates the transcription of different DNA sequences from those activated by the heterologous non-histone protein fraction. It is concluded that such non-histone protein fractions are capable of differentially augmenting transcription in vitro from DNA sequences of chromatin.
Keywords
Biochemistry, Genetics and Molecular Biology
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