The opsin family of proteins
Biochemical JournalPublished 15 September 1986Open access
J. B. C. Findlay, Darryl Pappin
Citations243
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TL;DR
The purpose of this review is to compare the structural and functional properties of a group of proteins that share the feature of being able to respond to light energy by way of a bound retinoid chromophore.
Keywords
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NatureLateral diffusion of rhodopsin in the photoreceptor membrane
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ScienceStructure of the GDP Domain of EF-Tu and Location of the Amino Acids Homologous to <i>ras</i> Oncogene Proteins
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NatureLight- and GTP-regulated interaction of GTPase and other proteins with bovine photoreceptor membranes
560 Citations1980Hermann Kühn
It is found that light induces profound changes in the interaction of rhodopsin kinase11, GTPase, and other proteins with the photoreceptor membrane that are reversible in the dark, are strongly influenced by GTP, and are thought to be involved in the regulation of enzyme activity by light.
CellThe Drosophila ninaE gene encodes an opsin
545 Citations1985Joseph E. O’Tousa, Wolfgang Baehr +4 more
The Drosophila and vertebrate opsin genes are derived from a common ancestor; there is strong conservation of the retinal binding site and two other regions and the predicted protein secondary structure strikingly resembles that of mammalian opsins.
European Biophysics JournalThe structure of bovine rhodopsin
519 Citations1983Paul A. Hargrave, J. Hugh McDowell +6 more
The sequence of most of the cyanogen bromide peptides has been completed, forming the basis for the prediction of the secondary structure of the protein and how it may be arranged in the disk membrane.
Proceedings of the National Academy of SciencesIsolation and nucleotide sequence of the gene encoding human rhodopsin.
499 Citations1984Jeremy Nathans, David S. Hogness
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CellIsolation and structure of a rhodopsin gene from D. melanogaster
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284 Citations1982Patrick Argos, Jayasimha Rao +1 more
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191 Citations1981Daniela Pietrobon, Giovanni Azzone +1 more
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185 Citations1979Michael L. Woodruff, M D Bownds
The concentration of guanosine 3',5'-cyclic monophosphate (cyclic GMP) has been examined in suspensions of freshly isolated frog rod outer segments using conditions which previously have been shown to maintain the ability of outer segments to perform a light-induced permeability change.
Journal of Molecular BiologyTransient and linear dichroism studies on bacteriorhodopsin: Determination of the orientation of the 568 nm all-trans retinal chromophore
174 Citations1977Maarten P. Heyn, Richard J. Cherry +1 more
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170 Citations1979Mike Rehorek, Maarten P. Heyn
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170 Citations1979Gerhard E. Gerber, Robert J. Anderegg +4 more
The sequence of 102 amino acid residues from the NH2 terminus and that of 39 amino acid residue from the COOH terminus of bacteriorhodopsin have been determined by a judicious combination of mass spectrometric peptide sequencing and automated Edman degradation.
Journal of Biological ChemistryMagnitude of increase in retinal cGMP metabolic flux determined by 18O incorporation into nucleotide alpha-phosphoryls corresponds with intensity of photic stimulation.
169 Citations1983N D Goldberg, A. Ames +2 more
It is postulated that increases in cGMP metabolic flux rather than changes incGMP steady state levels are integral to phototransduction by a mechanism that involves the coupling of cG MP synthesis and/or hydrolysis to either the release of calcium from disc membranes or the inhibition of Na+ conductance by the photoreceptor membrane.
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168 Citations1975Maarten P. Heyn, Paul J. Bauer +1 more
This CD effect appears to be a sensitive probe to study changes in protein mobility and in protein-protein interactions and is suggested that DMSO increases the protein mobility in the membrane.
Proceedings of the National Academy of SciencesGTPase of bovine rod outer segments: the amino acid sequence of the alpha subunit as derived from the cDNA sequence.
168 Citations1985Kimio Yatsunami, H. Gobind Khorana
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Proceedings of the National Academy of SciencesFourier transform infrared difference spectroscopy of bacteriorhodopsin and its photoproducts.
166 Citations1982Kimberly A. Bagley, Gavin Dollinger +3 more
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158 Citations1985Peter Gierschik, Juan Codina +3 more
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157 Citations1980Janet K. Wang, Jenny McDowell +1 more
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152 Citations1978Richard J. Cherry, Ulrike Müller +2 more
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148 Citations1981Hagan Bayley, Kuo‐Sen Huang +4 more
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146 Citations1985Rodger Bodoia, Peter B. Detwiler
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146 Citations1976John Bridgen, Ian D. Walker
No evidence of chain heterogeneity was found by radiochemical peptide mapping and sequence analysis of peptides containing lysine residues indicating that all protein chains of purple membrane are very similar or identical.
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142 Citations1977David F. O’Brien, Larry F. Costa +1 more
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Photochemistry and PhotobiologyON THE MECHANISM OF WAVELENGTH REGULATION IN VISUAL PIGMENTS
138 Citations1985Hiroko Kakitani, Toshiaki Kakitani +2 more
The theoretical basis for the “external point charge” model proposed previously, i.e. mechanism (1), appears to provide the crucial interaction in the protein induced spectral shifts of bovine rhodopsin.
Proceedings of the National Academy of SciencesOn the protein (tyrosine)-chromophore (protonated Schiff base) coupling in bacteriorhodopsin.
138 Citations1984JANE H. HANAMOTO, P. Dupuis +1 more
The kinetics of formation of both the tyrosinate ion and the deprotonated Schiff base (M412) are studied simultaneously at different pH values (7-11) and temperatures (5-25 degrees C).
Proceedings of the National Academy of SciencesControl of transmembrane ion fluxes to select halorhodopsin-deficient and other energy-transduction mutants of Halobacterium halobium.
135 Citations1982Elena N. Spudich, John L. Spudich
A selection method for mutants altered in the generation and regulation of transmembrane ion flux in Halobacterium halobium using alterations in the halobacterial rhodopsin to select for mutants that escape cytoplasmic acidification.
The Journal of General PhysiologyRestrictions on rotational and translational diffusion of pigment in the membranes of a rhabdomeric photoreceptor.
134 Citations1977Timothy H. Goldsmith, Rüdiger Wehner
Quantitative comparison of photodichroism with mathematical models indicates that the pigment absorption vectors are aligned within +/- 50 degrees of the microvillar axes and are tilted into the surface of the membrane at an average value of about 20 degrees, suggesting that the bifunctional reagent quiets some molecular motion that is present after treatment with formaldehyde.
CellAn opsin gene expressed in only one photoreceptor cell type of the Drosophila eye
132 Citations1986Alan F. Cowman, Charles S. Zuker +1 more
An opsin gene from D. melanogaster that is expressed specifically in photoreceptor cell 8 of the Drosophila compound eye is isolated and is 67% homologous to the ninaE opsin, which is expressed inPhotoreceptor cells 1-6.
BiochemistryEffects of lipid environment on the light-induced conformational changes of rhodopsin. 2. Roles of lipid chain length, unsaturation, and phase state
131 Citations1985Patricia A. Baldwin, Wayne L. Hubbell
By incorporation of rhodopsin into a series of phosphatidylcholines of defined composition, this work has determined the properties of the lipid environment that are responsible for the altered spectral behavior.
Biophysical JournalThe quantum efficiency of proton pumping by the purple membrane of Halobacterium halobium
131 Citations1980Rajni Govindjee, Thomas G. Ebrey +1 more
The observation that the proton relaxation time is much longer than the photochemical cycling time suggest that the protons are pumped across the membrane and there is no contribution as a result of reversible binding and release of protons on just one side of the membrane.
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